Querzoli P, Albonico G, Ferretti S, Rinaldi R, Magri E, Indelli M, Nenci I
Istituto de Anatomia, Università di Ferrara, Italy.
J Clin Pathol. 1996 Nov;49(11):926-30. doi: 10.1136/jcp.49.11.926.
To determine cell proliferation in infiltrating breast carcinomas.
Using the MIB-1 monoclonal antibody, the proliferation index was measured in paraffin wax sections of 871 breast cancers. The MIB-1 proliferation index was compared with other markers of disease progression: size, lymph node status, histotype, oestrogen and progesterone receptor status, expression of p53 and Neu, and DNA ploidy. All parameters were measured using image analysis. In 347 tumours, the MIB-1 and Ki-67 proliferation indexes were compared. Follow up data were available for 170 cases (median 66.5 months).
Of the tumours, 314 (36%) had a high proliferation index. The MIB-1 proliferation index was correlated directly with size, nodal status, overexpression of p53 and Neu, and the DNA index; and inversely with oestrogen and progesterone receptor status. The correlation between MIB-1 and Ki-67 proliferation indexes was statistically significant. In patients with pT1 tumours, a low proliferation index correlated with a longer relapse-free interval and overall survival; node negative patients with a low proliferation index had a longer overall survival.
The MIB-1 proliferation index is a reliable, practical and useful method of measuring proliferative activity and is an important predictor of clinical behaviour.
确定浸润性乳腺癌中的细胞增殖情况。
使用MIB-1单克隆抗体，在871例乳腺癌的石蜡切片中测量增殖指数。将MIB-1增殖指数与疾病进展的其他标志物进行比较：肿瘤大小、淋巴结状态、组织学类型、雌激素和孕激素受体状态、p53和Neu的表达以及DNA倍体。所有参数均使用图像分析进行测量。在347例肿瘤中，比较了MIB-1和Ki-67增殖指数。有170例患者的随访数据（中位数为66.5个月）。
在这些肿瘤中，314例（36%）具有高增殖指数。MIB-1增殖指数与肿瘤大小、淋巴结状态、p53和Neu的过表达以及DNA指数直接相关；与雌激素和孕激素受体状态呈负相关。MIB-1和Ki-67增殖指数之间的相关性具有统计学意义。在pT1肿瘤患者中，低增殖指数与较长的无复发生存期和总生存期相关；低增殖指数的淋巴结阴性患者具有较长的总生存期。
MIB-1增殖指数是一种可靠、实用且有用的测量增殖活性的方法，是临床行为的重要预测指标。
