Ma L, Zablow L, Kandel E R, Siegelbaum S A
Department of Pharmacology, Columbia University, New York, New York 10032, USA.
Nat Neurosci. 1999 Jan;2(1):24-30. doi: 10.1038/4525.
Long-term forms of synaptic plasticity that may underlie learning and memory have been suggested to depend on changes in the number of synapses between presynaptic and postsynaptic neurons. Here we have investigated a form of synaptic plasticity in cultures of hippocampal CA3 and CA1 neurons related to the late phase of long-term potentiation, which depends on cAMP and protein synthesis. Using the fluorescent dye FM 1-43 to label active presynaptic terminals, we find that a membrane permeable analog of cAMP enhances the number of active presynaptic terminals and that this effect requires protein synthesis.
长期的突触可塑性形式可能是学习和记忆的基础，有人认为它依赖于突触前神经元和突触后神经元之间突触数量的变化。在这里，我们研究了海马CA3和CA1神经元培养物中与长时程增强后期相关的一种突触可塑性形式，长时程增强后期依赖于cAMP和蛋白质合成。使用荧光染料FM 1-43标记活跃的突触前终末，我们发现cAMP的一种膜通透性类似物增加了活跃突触前终末的数量，并且这种效应需要蛋白质合成。
