Blasi F, Riccio M, Brogi A, Strazza M, Taddei M L, Romagnoli S, Luddi A, D'Angelo R, Santi S, Costantino-Ceccarini E, Melli M
Dipartimento Biologia Evoluzionistica Sperimentale, Università di Bologna, Italy.
Biol Chem. 1999 Feb;380(2):259-64. doi: 10.1515/BC.1999.034.
The RT-PCR analysis of RNA from progenitor and differentiated primary rat oligodendrocytes, and from the oligodendrocyte CG-4 cell line, shows the presence of the IL-1beta mRNA, the type I IL-1beta receptor and the IL-1 receptor accessory protein in these cells. In situ hybridization of a rat IL-1beta probe to primary progenitor and differentiated rat oligodendrocytes results in a positive signal. The double hybridization of the IL-1beta probe, together with an oligodendrocyte-specific differentiation marker, to sections of postnatal rat brain at different stages of differentiation is also positive. The double immuno-labelling technique utilized indicates coincidence of the signals on the brain slices. The results show that IL-1beta mRNA is constitutively expressed in rat brain oligodendrocytes from 1 day after birth onward. In agreement with this observation, CG-4 cells, primary progenitor and differentiated rat oligodendrocytes are positively stained by antibodies against IL-1beta. Postnatal brain slices from 1 and 4 day old and adult rats, labelled with a double immunofluorescence technique, are also stained by antibodies against IL-1beta. This signal coincides with that of antibodies against oligodendrocyte-specific surface markers. We conclude that IL-1beta is constitutively expressed in rat brain progenitor and differentiated oligodendrocytes.
对原代大鼠少突胶质前体细胞和分化的少突胶质细胞以及少突胶质细胞CG - 4细胞系的RNA进行逆转录聚合酶链反应（RT-PCR）分析，结果显示这些细胞中存在白细胞介素-1β（IL-1β）信使核糖核酸（mRNA）、I型IL-1β受体和IL-1受体辅助蛋白。用大鼠IL-1β探针与原代少突胶质前体细胞和分化的大鼠少突胶质细胞进行原位杂交，结果呈阳性。将IL-1β探针与少突胶质细胞特异性分化标志物一起对出生后不同分化阶段的大鼠脑切片进行双重杂交，结果也呈阳性。所采用的双重免疫标记技术表明脑切片上的信号相互吻合。结果显示，从出生后1天起，IL-1β mRNA在大鼠脑少突胶质细胞中持续表达。与此观察结果一致，CG-4细胞、原代少突胶质前体细胞和分化的大鼠少突胶质细胞被抗IL-1β抗体阳性染色。用双重免疫荧光技术标记的1日龄、4日龄和成年大鼠的出生后脑切片也被抗IL-1β抗体染色。该信号与抗少突胶质细胞特异性表面标志物抗体的信号相吻合。我们得出结论，IL-1β在大鼠脑少突胶质前体细胞和分化的少突胶质细胞中持续表达。
