Matsubara S, Yamada T, Minakami H, Watanabe T, Takizawa T, Sato I
Department of Obstetrics and Gynecology, Jichi Medical School, Kawachi-gun, Tochigi, Japan.
Placenta. 1999 Mar-Apr;20(2-3):185-8. doi: 10.1053/plac.1998.0366.
The purpose of this study was to examine evidence for the presence of activated leukocytes in the fetal membranes from patients with preterm delivery. Polymorphonuclear leukocytes in fetal membranes from seven patients with preterm delivery (26-32 weeks of gestation) were analysed using transmission electron microscopy and ultrastructural enzyme-histochemistry for peroxidase and alkaline phosphatase. A large number of leukocytes accumulated in the fetal membranes from preterm deliveries. Phagosome, phagocytosis of cell debris, attachment of primary granules to the phagosomal membrane and cell surface projections were observed in fetal membrane leukocytes from preterm delivery but not in peripheral blood leukocytes. Peroxidase and alkaline phosphatase activity was demonstrated on the plasma membrane of the phagosomes. Morphological and enzyme-histochemical observation indicated that polymorphonuclear leukocytes in the fetal membrane in patients with preterm delivery were stimulated or activated. Such activated leukocytes may play a role in the pathophysiology or pathogenesis of preterm delivery.
本研究的目的是检验早产患者胎膜中存在活化白细胞的证据。使用透射电子显微镜以及过氧化物酶和碱性磷酸酶的超微结构酶组织化学方法，对7例早产患者（妊娠26 - 32周）胎膜中的多形核白细胞进行了分析。早产患者的胎膜中积聚了大量白细胞。在早产患者的胎膜白细胞中观察到了吞噬体、细胞碎片的吞噬作用、初级颗粒附着于吞噬体膜以及细胞表面突起，但在外周血白细胞中未观察到。在吞噬体的质膜上证实了过氧化物酶和碱性磷酸酶活性。形态学和酶组织化学观察表明，早产患者胎膜中的多形核白细胞受到了刺激或被激活。这种活化的白细胞可能在早产的病理生理学或发病机制中起作用。
