Arcuri Felice, Toti Paolo, Buchwalder Lynn, Casciaro Alessandra, Cintorino Marcella, Schatz Frederick, Rybalov Basya, Lockwood Charles J
Department of Human Pathology and Oncology, University of Siena, Italy.
Reprod Sci. 2009 May;16(5):453-61. doi: 10.1177/1933719108328609. Epub 2009 Jan 22.
Chorioamnionitis is a major cause of prematurity as well as perinatal morbidity and mortality. The present study observed a marked increase in immunohistochemical staining for Colony Stimulating Factor 2 (CSF2; also known as granulocyte macrophage-colony stimulating factor), a potent neutrophil and macrophage chemoattractant and activator, in the decidua of patients with CAM compared with controls (n = 8; P = .001). To examine the regulation of this CSF2, cultured decidual cells primed with estradiol (E2) or E2 plus medroxyprogesterone acetate, were exposed to tumor necrosis factor-alpha or interleukin-1beta and secreted CSF2 measured by ELISA. Levels of CSF2 in E2 plus MPA-treated cultures increased 18- and 245-fold following treatment with TNF or IL1B (n = 7, P < .05). Quantitative RT-PCR demonstrated parallel changes in mRNA levels. This study reveals that CSF2 is strongly expressed in decidua from patients with CAM and indicates TNF or IL1B as important regulators of CAM-related decidual leukocyte infiltration and activation.
绒毛膜羊膜炎是早产以及围产期发病率和死亡率的主要原因。本研究观察到，与对照组（n = 8；P = .001）相比，绒毛膜羊膜炎（CAM）患者蜕膜中集落刺激因子2（CSF2；也称为粒细胞巨噬细胞集落刺激因子）的免疫组化染色显著增加，CSF2是一种有效的中性粒细胞和巨噬细胞趋化因子及激活剂。为了研究这种CSF2的调节机制，用雌二醇（E2）或E2加醋酸甲羟孕酮预处理的培养蜕膜细胞，分别暴露于肿瘤坏死因子-α或白细胞介素-1β，并用酶联免疫吸附测定法检测分泌的CSF2。用肿瘤坏死因子（TNF）或白细胞介素-1β（IL1B）处理后，E2加醋酸甲羟孕酮处理的培养物中CSF2水平分别增加了18倍和245倍（n = 7，P < .05）。定量逆转录聚合酶链反应（RT-PCR）显示mRNA水平有平行变化。本研究表明，CSF2在CAM患者的蜕膜中强烈表达，并表明TNF或IL1B是CAM相关蜕膜白细胞浸润和激活的重要调节因子。
