Men D, McCarty R, Gold P E
Department of Psychology, University of Virginia, 102 Gilmer Hall, Charlottesville, Virginia, 22903, USA.
Neurobiol Learn Mem. 1999 May;71(3):289-300. doi: 10.1006/nlme.1998.3880.
Recent evidence suggests that release of acetylcholine (ACh) in the hippocampus is associated with performance on a spontaneous alternation task and with enhancement of that performance by systemic and central injections of glucose. The present study extended these findings by examining norepinephrine (NE) release in the hippocampus using in vivo microdialysis while rats were tested for spontaneous alternation performance with and without prior injections (ip) of glucose. Microdialysis samples were collected every 12 min and assayed for NE content by HPLC-ECD. Like ACh, NE release in hippocampus increased during spontaneous alternation testing. As in past experiments, administration of glucose (250 mg/kg) significantly enhanced alternation scores. However, glucose did not influence NE release either during behavioral testing or at rest. These findings contrast with prior evidence showing that glucose augments testing-related increases in ACh release. The findings suggest that norepinephrine is released within the hippocampus while rats are engaged in alternation performance. However, increased release of norepinephrine apparently does not contribute to the enhancement of alternation scores produced by glucose.
最近的证据表明，海马体中乙酰胆碱（ACh）的释放与自发交替任务的表现以及全身和中枢注射葡萄糖对该表现的增强有关。本研究通过在大鼠进行有无预先注射（腹腔注射）葡萄糖的自发交替表现测试时，使用体内微透析检查海马体中去甲肾上腺素（NE）的释放，扩展了这些发现。每12分钟收集一次微透析样本，并通过高效液相色谱 - 电化学检测法（HPLC - ECD）测定NE含量。与ACh一样，在自发交替测试期间海马体中的NE释放增加。与过去的实验一样，给予葡萄糖（250mg / kg）显著提高了交替得分。然而，葡萄糖在行为测试期间或休息时均未影响NE释放。这些发现与先前的证据形成对比，先前证据表明葡萄糖会增强与测试相关的ACh释放增加。这些发现表明，当大鼠进行交替表现时，去甲肾上腺素在海马体内释放。然而，去甲肾上腺素释放的增加显然对葡萄糖产生的交替得分增强没有贡献。
