Stathopoulos-Gerontides A, Guo J J, Cyert M S
Deptartment of Biological Sciences, Stanford University, Stanford, California 94305-5020 USA.
Genes Dev. 1999 Apr 1;13(7):798-803. doi: 10.1101/gad.13.7.798.
Calcineurin, a Ca2+/calmodulin dependent protein phosphatase, regulates Ca2+-dependent processes in a wide variety of cells. In the yeast, Saccharomyces cerevisiae, calcineurin effects Ca2+-dependent changes in gene expression through regulation of the Crz1p transcription factor. We show here that calcineurin dephosphorylates Crz1p and that this results in translocation of Crz1p to the nucleus. We identify a region of Crz1p that is required for calcineurin-dependent regulation of its phosphorylation, localization, and activity, and show that this region has significant sequence simlarity to a portion of NF-AT, a family of mammalian transcription factors whose localization is also regulated by calcineurin. Thus, the mechanism of Ca2+/calcineurin-dependent signaling shows remarkable conservation between yeast and mammalian cells.
钙调神经磷酸酶是一种依赖于Ca2+/钙调蛋白的蛋白磷酸酶，可调节多种细胞中依赖Ca2+的过程。在酵母酿酒酵母中，钙调神经磷酸酶通过调节Crz1p转录因子来影响基因表达中依赖Ca2+的变化。我们在此表明，钙调神经磷酸酶使Crz1p去磷酸化，这导致Crz1p易位至细胞核。我们鉴定出Crz1p的一个区域，该区域对于钙调神经磷酸酶依赖的其磷酸化、定位和活性调节是必需的，并表明该区域与NF-AT的一部分具有显著的序列相似性，NF-AT是一类哺乳动物转录因子家族，其定位也受钙调神经磷酸酶调节。因此，Ca2+/钙调神经磷酸酶依赖的信号传导机制在酵母和哺乳动物细胞之间表现出显著的保守性。
