Sorimachi K, Naora H, Akimoto K, Niwa A, Naora H
Department of Microbiology, Dokkyo University School of Medicine, Tochigi, Mibu, 321-02, Japan.
Cell Biol Int. 1998;22(5):351-7. doi: 10.1006/cbir.1998.0266.
Rat bone marrow-derived macrophages formed multinucleated giant cells spontaneously when cultured in slide glass chambers or when induced with the polyanion acetyl lignin. Nuclei in such cells tended to cluster in distinct rings. DNA fragmentation appeared to occur in multinucleated cells, as detected by 3' end-labeling. Southern blot analyses, using probes specific for nucleolar and non-nucleolar genes, indicated that chromatin DNA was fragmented whereas nucleolar DNA was relatively intact. Autoradiography revealed preservation, in multinucleated cells, of nucleoli into which radiolabeled uridine was incorporated. Multinucleated macrophages appeared to eventually fragment. Preserved integrity of nucleoli seems to be a feature of macrophage multinucleation, a process which apparently culminates in cell death.
大鼠骨髓来源的巨噬细胞在载玻片培养室中培养或用聚阴离子乙酰木质素诱导时会自发形成多核巨细胞。这些细胞中的细胞核倾向于聚集成不同的环。如通过3'末端标记检测到的，多核细胞中似乎发生了DNA片段化。使用针对核仁基因和非核仁基因的探针进行的Southern印迹分析表明，染色质DNA发生了片段化，而核仁DNA相对完整。放射自显影显示，在多核细胞中，核仁得以保留，放射性标记的尿苷被掺入其中。多核巨噬细胞最终似乎会发生片段化。核仁的完整性得以保留似乎是巨噬细胞多核化的一个特征，这一过程显然最终导致细胞死亡。
