Matsumoto H, Murakami Y, Horikoshi Y, Noguchi J, Habata Y, Kitada C, Hinuma S, Onda H, Fujino M
Pharmaceutical Discovery Division, Takeda Chemical Industries Ltd., Wadai-10, Tsukuba Ibaraki, 300-4293, Japan.
Biochem Biophys Res Commun. 1999 Apr 13;257(2):264-8. doi: 10.1006/bbrc.1999.0463.
We established a sensitive and specific two-site enzyme immunoassay (EIA) for prolactin-releasing peptide (PrRP) using two region-specific monoclonal antibodies. We investigated the tissue distribution and the plasma concentration of immunoreactive (ir-) PrRP in rats using this assay. Ir-PrRP was widely distributed in the central nervous system and pituitary gland. The highest concentration of ir-PrRP was found in the hypothalamus. In peripheral tissues, appreciable levels of ir-PrRP were found only in the adrenal gland. The mean plasma concentration of ir-PrRP was 0.13 +/- 0.01 fmol/ml (mean +/- SEM). In reverse-phase and gel-filtration high performance liquid chromatography, hypothalamic ir-PrRP eluted at a position identical to that of PrRP31 and PrRP20. On the other hand, ir-PrRP from the adrenal gland and plasma eluted only at the position of synthetic PrRP31, indicating that molecular forms of ir-PrRP in vivo differed among tissues.
我们使用两种区域特异性单克隆抗体建立了一种灵敏且特异的催乳素释放肽（PrRP）双位点酶免疫测定法（EIA）。我们用该测定法研究了大鼠体内免疫反应性（ir-）PrRP的组织分布和血浆浓度。ir-PrRP广泛分布于中枢神经系统和垂体。在下丘脑中发现ir-PrRP浓度最高。在外周组织中，仅在肾上腺中发现了可观水平的ir-PrRP。ir-PrRP的平均血浆浓度为0.13±0.01 fmol/ml（平均值±标准误）。在反相和凝胶过滤高效液相色谱中，下丘脑ir-PrRP在与PrRP31和PrRP20相同的位置洗脱。另一方面，来自肾上腺和血浆的ir-PrRP仅在合成PrRP31的位置洗脱，表明体内ir-PrRP的分子形式在不同组织间存在差异。
