Brubacher-Kauffmann S, Maréchal-Drouard L, Cosset A, Dietrich A, Duchêne A M
Institut de Biologie Moléculaire des Plantes du CNRS (UPR 0406), Université Louis Pasteur, 12 rue du Général Zimmer, 67084 Strasbourg Cedex, France.
Nucleic Acids Res. 1999 May 1;27(9):2037-42. doi: 10.1093/nar/27.9.2037.
In potato ( Solanum tuberosum ) mitochondria, about two-thirds of the tRNAs are encoded by the mitochondrial genome and one-third is imported from the cytosol. In the case of tRNAGly isoacceptors, a mitochondrial-encoded tRNAGly(GCC) was found in potato mitochondria, but this is likely to be insufficient to decode the four GGN glycine codons. In this work, we identified a cytosolic tRNAGly(UCC), which was found to be present in S.tuberosum mitochondria. The cytosolic tRNAGly(CCC) was also present in mitochondria, but to a lesser extent. By contrast, the cytosolic tRNAGly(GCC) could not be detected in mitochondria. This selective import of tRNAGly isoacceptors into S. tuberosum mitochondria raises further questions about the mechanism under-lying the specificity of the import process.
在马铃薯(Solanum tuberosum)线粒体中,约三分之二的tRNA由线粒体基因组编码,三分之一从细胞质中导入。就甘氨酸tRNA同工受体而言,在马铃薯线粒体中发现了一种线粒体编码的tRNAGly(GCC),但这可能不足以解码四个GGN甘氨酸密码子。在这项研究中,我们鉴定出一种细胞质tRNAGly(UCC),发现它存在于马铃薯线粒体中。细胞质tRNAGly(CCC)也存在于线粒体中,但含量较少。相比之下,在线粒体中未检测到细胞质tRNAGly(GCC)。甘氨酸tRNA同工受体向马铃薯线粒体的这种选择性导入,进一步引发了关于导入过程特异性背后机制的问题。