Niku-Paavola M L, Laitila A, Mattila-Sandholm T, Haikara A
VTT Biotechnology and Food Research, Espoo, Finland.
J Appl Microbiol. 1999 Jan;86(1):29-35. doi: 10.1046/j.1365-2672.1999.00632.x.
New types of antimicrobial compounds were identified in the culture filtrate of Lactobacillus plantarum VTT E-78076. Activity was detected in the low molecular mass fraction separated by gel chromatography. This fraction totally inhibited the growth of the Gram-negative test organism, Pantoea agglomerans (Enterobacter agglomerans) VTT E-90396. Characteristic compounds from this fraction were identified by GC/MS-analysis and the identification was confirmed using pure commercial reference compounds in identical chromatographs and in antimicrobial tests. The active fraction included benzoic acid (CAS 65-85-0), 5-methyl-2,4-imidazolidinedione (CAS 616-03-5, methylhydantoin), tetrahydro-4-hydroxy-4-methyl-2H- pyran-2-one (CAS 674-26-0, mevalonolactone) and 3-(2-methylpropyl)-2,5-piperazinedione (CAS 5845-67-0, cyclo(glycyl-L-leucyl)). These compounds in concentrations of 10 ppm inhibited growth of the test organism by 10-15% when acting separately, but 100% when all were applied together with 1% lactic acid. The inhibition was 40% by 1% lactic acid alone. The compounds were also active against Fusarium avenaceum (Gibberella avenacea) VTT-D-80147. The inhibition was 10-15% by separate compounds in concentrations of 10 ppm and maximally 20% in combinations. Fungal growth was not inhibited by lactic acid. Inhibition by unfractionated Lact. plantarum culture filtrate was 37% and by the low molecular mass fraction, 27%.
在植物乳杆菌VTT E-78076的培养滤液中鉴定出了新型抗菌化合物。通过凝胶色谱分离的低分子量部分检测到了活性。该部分完全抑制了革兰氏阴性测试菌成团泛菌（聚团肠杆菌）VTT E-90396的生长。通过气相色谱/质谱分析鉴定了该部分的特征化合物，并使用相同色谱仪中的纯商业参考化合物以及抗菌试验对鉴定结果进行了确认。活性部分包括苯甲酸（CAS 65-85-0）、5-甲基-2,4-咪唑啉二酮（CAS 616-03-5，甲基乙内酰脲）、四氢-4-羟基-4-甲基-2H-吡喃-2-酮（CAS 674-26-0，甲羟戊酸内酯）和3-(2-甲基丙基)-2,5-哌嗪二酮（CAS 5845-67-0，环（甘氨酰-L-亮氨酰））。这些化合物单独作用时，浓度为10 ppm可使测试菌的生长抑制10-15%，但与1%乳酸一起使用时，全部化合物共同作用可使生长抑制100%。单独使用1%乳酸时抑制率为40%。这些化合物对燕麦镰刀菌（燕麦赤霉）VTT-D-80147也有活性。单独的化合物浓度为10 ppm时抑制率为10-15%，组合使用时最大抑制率为20%。乳酸对真菌生长没有抑制作用。未分级的植物乳杆菌培养滤液的抑制率为37%，低分子量部分的抑制率为27%。
