Department of MBBT, Tezpur University, Napaam-784028, Assam, India.
Int Microbiol. 2024 Feb;27(1):179-202. doi: 10.1007/s10123-023-00376-8. Epub 2023 Jun 1.
Beneficial and pathogenic microbes coexist in the vaginal canal, where a diminishing population of lactic acid bacteria may cause recurring urogenital infections. Probiotic bacteria Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus vaginalis, and pathogenic microbes Enterococcus faecalis, Enterobacter cloacae, Shigella sp., Staphylococcus epidermidis, and Escherichia fergusonii were isolated from vaginal swabs. Lactobacillus sp. and their probiotic culture free supernatant (PCFS) inhibited the growth of the above-mentioned urogenital pathogens. L. crispatus produced both lactic acid and hydrogen peroxide, exhibiting the best antimicrobial potential against the studied pathogens. Lyophilized L. crispatus had a shelf life of 12 months and the lyophilized PCFS also retained its antibacterial property with a minimum inhibition concentration of 1 μg/μL. Carboxy-methyl cellulose-alginate, a green alternative to super-absorbent polymers, was encapsulated with L. crispatus cells. The probiotic in its encapsulated state retained its viability for 21 days, and the bead showed 30% solvent absorptive capacity. PCFS-laced non-woven fabric displayed antibacterial property with no change in its physicochemical properties. These probiotic and postbiotic formulations have excellent prophylactic potential for urogenital infections. Such formulations can be exploited as additives in sanitary suppositories to enhance vaginal health.
有益和致病微生物共存于阴道中，其中乳酸杆菌数量减少可能导致反复发生泌尿生殖系统感染。从阴道拭子中分离出益生菌细菌乳杆菌、加氏乳杆菌、阴道乳杆菌和致病性微生物粪肠球菌、阴沟肠杆菌、志贺氏菌、表皮葡萄球菌和弗格森埃希氏菌。乳杆菌属及其益生菌培养上清液（PCFS）抑制上述泌尿生殖系统病原体的生长。L. crispatus 既能产生乳酸又能产生过氧化氢，对研究中的病原体表现出最好的抗菌潜力。冻干的 L. crispatus 保质期为 12 个月，冻干的 PCFS 也保留了其抗菌性能，最小抑制浓度为 1μg/μL。羧甲基纤维素-藻酸盐是超级吸水聚合物的绿色替代品，用 L. crispatus 细胞包封。封装状态下的益生菌保持 21 天的活力，珠粒显示 30%的溶剂吸收能力。载有 PCFS 的无纺布显示出抗菌性能，其物理化学性质没有变化。这些益生菌和后生菌制剂对泌尿生殖系统感染具有极好的预防潜力。这些制剂可作为卫生栓剂中的添加剂，以增强阴道健康。
