Chen G, Hanson C L, Dunbar R L, Ebner T J
Department of Neurosurgery, University of Minnesota, Minneapolis, Minnesota 55455, USA.
J Neurophysiol. 1999 Apr;81(4):1992-8. doi: 10.1152/jn.1999.81.4.1992.
A novel form of spreading acidification and depression in the rat cerebellar cortex was imaged in vivo using the pH-sensitive dye, Neutral red. Surface stimulation evoked an initial beam of increased fluorescence (i.e., decreased pH) that spread rostrally and caudally across the folium and into neighboring folia. A transient but marked suppression in the excitability of the parallel fiber-Purkinje cell circuitry accompanied the spread. Characteristics differentiating this phenomenon from the spreading depression of Leao include: high speed of propagation on the surface (average of 450 microm/s), stable extracellular DC potential, no change in blood vessel diameter, and repeatability at short intervals. This propagating acidification constitutes a previously unknown class of neuronal processing in the cerebellar cortex.
利用pH敏感染料中性红在体内对大鼠小脑皮质中一种新形式的扩展性酸化和抑制进行了成像。表面刺激引发了一束初始的荧光增强（即pH降低），该荧光束向头端和尾端扩散，穿过小叶并进入相邻小叶。在扩散过程中，平行纤维-浦肯野细胞回路的兴奋性出现短暂但显著的抑制。将这种现象与Leao的扩展性抑制区分开来的特征包括：在表面的传播速度快（平均450微米/秒）、细胞外直流电位稳定、血管直径无变化以及短时间间隔内的可重复性。这种传播性酸化构成了小脑皮质中一类以前未知的神经元活动过程。
