Sarr A D, Popper S, Thior I, Hamel D J, Sankalé J L, Siby T, Marlink R, Essex M, Mboup S, Kanki P
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115-6017, USA.
J Hum Virol. 1999 Jan-Feb;2(1):45-51.
To explore and compare the relations between proviral DNA load and CD4+ lymphocyte counts in both HIV-2 monotypic and HIV dual infection.
STUDY DESIGN/METHODS: In Dakar, Senegal, where the HIV-1 and HIV-2 epidemics overlap, serum and peripheral blood mononuclear cell (PBMC) DNA samples were collected from registered female sex workers and hospitalized patients. Sera were evaluated for reactivity to antigens of HIV-1 and HIV-2 by immunoblot; dual reactivity was confirmed with recombinant envelope peptides for HIV-1 and HIV-2. These samples were then subjected to HIV-1 and HIV-2 proviral DNA polymerase chain reaction (PCR). To evaluate the HIV-2 cellular proviral DNA loads, a quantitative competitive PCR (QC-PCR) was developed using nested primers to amplify the gag region of HIV-2. This assay used an internal competitor generated by inserting 25 bp in the first-round PCR target sequence. T-lymphocyte subset counts were estimated by flow cytometry for both HIV-2 monotypic and dually infected persons.
35 HIV-2-infected and 33 dually seroreactive samples were evaluated in this study. The CD4+ lymphocyte counts were similar in both groups, with mean values of 449 +/- 390 cells/mm3 for the HIV-2 monotypic infected persons and 476 +/- 308 cells/mm3 among the dually infected persons. However, the median proviral loads differed significantly, with those in the HIV-2 group ranging from 63.2 to 669.8 copies/10(5) CD4+ cells and demonstrating an inverse correlation with CD4+ lymphocyte count. The HIV dually infected persons showed less variation in viral load, ranging from 9.9 to 43.3 copies/10(5) CD4+ cells. Among the HIV dually infected persons, low HIV-2 proviral load was correlated with low CD4+ lymphocyte counts.
The HIV-2 proviral loads in HIV dually infected persons were significantly lower than those in HIV-2 monotypically infected individuals (P < .0001), despite comparable CD4+ lymphocyte counts. These results suggest that different HIV-2 proviral dynamics prevail in HIV dual infection.
探讨并比较HIV - 2单一感染和HIV双重感染中前病毒DNA载量与CD4 + 淋巴细胞计数之间的关系。
研究设计/方法：在塞内加尔达喀尔，HIV - 1和HIV - 2疫情重叠地区，从登记在册的女性性工作者和住院患者中采集血清和外周血单核细胞（PBMC）DNA样本。通过免疫印迹法评估血清对HIV - 1和HIV - 2抗原的反应性；用HIV - 1和HIV - 2的重组包膜肽确认双重反应性。然后对这些样本进行HIV - 1和HIV - 2前病毒DNA聚合酶链反应（PCR）。为评估HIV - 2细胞前病毒DNA载量，开发了一种定量竞争性PCR（QC - PCR），使用巢式引物扩增HIV - 2的gag区域。该检测使用通过在第一轮PCR靶序列中插入25 bp产生的内部竞争者。通过流式细胞术估计HIV - 2单一感染和双重感染患者的T淋巴细胞亚群计数。
本研究评估了35份HIV - 2感染样本和33份双重血清反应阳性样本。两组的CD4 + 淋巴细胞计数相似，HIV - 2单一感染患者的平均值为449±390个细胞/mm³，双重感染患者为476±308个细胞/mm³。然而，前病毒载量中位数差异显著，HIV - 2组的前病毒载量为63.2至669.8拷贝/10⁵ CD4 + 细胞，并与CD4 + 淋巴细胞计数呈负相关。HIV双重感染患者的病毒载量变化较小，为9.9至43.3拷贝/10⁵ CD4 + 细胞。在HIV双重感染患者中，低HIV - 2前病毒载量与低CD4 + 淋巴细胞计数相关。
尽管CD4 + 淋巴细胞计数相当，但HIV双重感染患者的HIV - 2前病毒载量显著低于HIV - 2单一感染个体（P <.0001）。这些结果表明，HIV双重感染中存在不同的HIV - 2前病毒动态。
