HIV-1感染患者与HIV-2感染患者之间前病毒DNA载量的差异。

Differences in proviral DNA load between HIV-1- and HIV-2-infected patients.

作者信息

Gueudin Marie, Damond Florence, Braun Joséphine, Taïeb Audrey, Lemée Véronique, Plantier Jean-Christophe, Chêne Geneviève, Matheron Sophie, Brun-Vézinet Françoise, Simon François

机构信息

Laboratoire de Virologie, CHU Charles Nicolle Rouen, France.

出版信息

AIDS. 2008 Jan 11;22(2):211-5. doi: 10.1097/QAD.0b013e3282f42429.

DOI:10.1097/QAD.0b013e3282f42429

PMID:18097223

Abstract

INTRODUCTION

The lesser pathogenicity of HIV-2 relative to HIV-1 is generally attributed to its slower replication. To compare the amounts of total HIV DNA during human HIV-1 and HIV-2 infection, we developed a quantitative real-time PCR method with a unique external quantification standard based on a single plasmid harboring both the HIV-1 and the HIV-2 LTR.

METHODS

Viral DNA load was compared between 40 HIV-1-infected and 42 HIV-2-infected antiretroviral-naive patients.

RESULTS

The difference between HIV-1 and HIV-2 proviral DNA load was highly significant in patients with CD4 cell counts > 500 cells/microl [HIV-1: n = 14; median, 2.5; interquartile range (IQR), 2.1-2.7; HIV-2: n = 22, median, 1.6; IQR, 1.0-2.0] and in patients with CD4 cell counts between 300 cells/microl and 500 cells/microl (HIV-1: n = 12; median, 2.7; IQR, 2.3-2.8; HIV-2: n = 11; median, 2.0; IQR, 1.0-2.4). Too few HIV-2-infected patients had CD4 cell counts < 300 cells/microl to detect a significant difference but DNA values were again lower in HIV-2-infected patients (HIV-1: n = 14; median, 2.9; IQR, 2.2-3.2; HIV-2: n = 9; median, 2.7; IQR, 2.2-3.3).

CONCLUSIONS

These differences are in line with the natural histories of the two infections and show that HIV-2 infection is a valid model for studying the pathophysiology of HIV infection in general.

摘要

引言

HIV-2相对于HIV-1致病性较低，这通常归因于其复制速度较慢。为了比较人类HIV-1和HIV-2感染期间总HIV DNA的量，我们开发了一种基于携带HIV-1和HIV-2长末端重复序列（LTR）的单个质粒的独特外部定量标准的定量实时PCR方法。

方法

比较了40例未接受抗逆转录病毒治疗的HIV-1感染患者和42例未接受抗逆转录病毒治疗的HIV-2感染患者的病毒DNA载量。

结果

在CD4细胞计数>500个/微升的患者中（HIV-1：n = 14；中位数，2.5；四分位间距（IQR），2.1 - 2.7；HIV-2：n = 22，中位数，1.6；IQR，1.0 - 2.0）以及CD4细胞计数在300个/微升至500个/微升之间的患者中（HIV-1：n = 12；中位数，2.7；IQR，2.3 - 2.8；HIV-2：n = 11；中位数，2.0；IQR，1.0 - 2.4），HIV-1和HIV-2前病毒DNA载量的差异非常显著。CD4细胞计数<300个/微升的HIV-2感染患者太少，无法检测到显著差异，但HIV-2感染患者的DNA值再次较低（HIV-1：n = 14；中位数，2.9；IQR，2.2 - 3.2；HIV-2：n = 9；中位数，2.7；IQR，2.2 - 3.3）。