人类反应性星形胶质细胞中神经节苷脂GD3的证实。

Demonstration of ganglioside GD3 in human reactive astrocytes.

作者信息

Kawai K, Watarai S, Takahashi H, Ishizu H, Fukai K, Tanabe Y, Yokota O, Kuroda S

机构信息

Department of Neuropsychiatry, Okayama University Medical School, Japan.

出版信息

Psychiatry Clin Neurosci. 1999 Feb;53(1):79-82. doi: 10.1046/j.1440-1819.1999.00475.x.

DOI:10.1046/j.1440-1819.1999.00475.x

PMID:10201289

Abstract

Astrocytes are the cells that actively participate in the process of lesion repair in the central nervous system (CNS), and reactive astrocytosis of varying degrees becomes apparent with time in any pathological condition occurring in the normally developed postnatal CNS. Ganglioside GD3 (II3a(NeuAca2-8NeuAc)-LacCer, GD3) in reactive astrocytes from autopsied patients with Creutzfeldt-Jakob disease (CJD) and old cerebral infarction was investigated immunocytochemically, using mouse IgM anti-GD3 monoclonal antibody (DSG-1). Reactive astrocytes in CJD and cerebral infarction demonstrated GD3-immunoreactivity within the cytoplasm. Normal astrocytes were negative. The present data raise the possibility that GD3 in reactive astrocytes has biological implications for the properties of the cells, such as cellular motility.

摘要

星形胶质细胞是积极参与中枢神经系统（CNS）损伤修复过程的细胞，在正常发育的出生后CNS中发生的任何病理状况下，不同程度的反应性星形胶质细胞增生会随着时间推移而变得明显。使用小鼠IgM抗GD3单克隆抗体（DSG-1），通过免疫细胞化学方法研究了来自克雅氏病（CJD）尸检患者和陈旧性脑梗死患者的反应性星形胶质细胞中的神经节苷脂GD3（II3a(NeuAca2-8NeuAc)-LacCer，GD3）。CJD和脑梗死中的反应性星形胶质细胞在细胞质内显示出GD3免疫反应性。正常星形胶质细胞呈阴性。目前的数据增加了一种可能性，即反应性星形胶质细胞中的GD3对细胞特性（如细胞运动性）具有生物学意义。