反应性星形胶质细胞中GD3神经节苷脂的出现——大鼠脑的免疫细胞化学研究

Occurrence of GD3 ganglioside in reactive astrocytes--an immunocytochemical study in the rat brain.

作者信息

Kawai K, Kuroda S, Watarai S, Takahashi H, Ikuta F

机构信息

Department of Neuropsychiatry, Okayama University Medical School, Japan.

出版信息

Neurosci Lett. 1994 Jun 20;174(2):225-7. doi: 10.1016/0304-3940(94)90027-2.

DOI:10.1016/0304-3940(94)90027-2

PMID:7970185

Abstract

Immunocytochemical study of ganglioside GD3 (II3 alpha(NeuAc alpha 2-8NeuAc)-LacCer) was performed in the cold lesions produced in the cerebral cortex of the rat brain, using mouse IgM anti-GD3 monoclonal antibody (DSG-1). Seven and 15 days after cold lesioning, GD3-like immunoreactivity was observed in reactive astrocytes. Thirty and 50 days after cold lesioning, GD3-like immunoreactivity was observed in the cells that formed glial scars. Normal astrocytes were not immunoreactive. Therefore, it is possible that GD3 may play an important role in the astrocytic functions required for the process of repair of edematous lesions in the central nervous system.

摘要

使用小鼠IgM抗GD3单克隆抗体（DSG-1），对大鼠脑皮质冷损伤部位进行了神经节苷脂GD3（II3α(NeuAcα2-8NeuAc)-LacCer）的免疫细胞化学研究。冷损伤后7天和15天，在反应性星形胶质细胞中观察到GD3样免疫反应性。冷损伤后30天和50天，在形成胶质瘢痕的细胞中观察到GD3样免疫反应性。正常星形胶质细胞无免疫反应性。因此，GD3可能在中枢神经系统水肿性损伤修复过程所需的星形胶质细胞功能中发挥重要作用。