Ishihara O, Saitoh M, Kinoshita K, Takanashi H, Arai Y, Kitagawa H
Department of Obstetrics & Gynecology, Saitama Medical Center, Saitama Medical School, Kawagoe, Japan.
Acta Obstet Gynecol Scand. 1999 Apr;78(4):321-5.
To investigate the role of interleukin-1 related substances in amniotic fluid in normal term labor without intrauterine infection.
Amniotic fluid samples were collected from forty-one patients with various backgrounds. A novel pre-assay treatment using Frigen II was introduced to improve the recovery rates of cytokines, i.e., interleukin-1alpha, interleukin-1beta and interleukin-1 receptor antagonist, prior to ELISA assay. Urine samples from newborn infants were also tested.
The concentrations of interleukin-1alpha, interleukin-1beta and interleukin-1ra were significantly higher in samples from normal vaginal delivery. The higher levels of interleukin-1alpha were also observed in samples from preterm labor without infection. Preterm infants produced more of interleukin-1 receptor antagonist in urine compared with term infants.
This study provides evidence of the possible involvement of interleukin-1 related substances in labor without signs of infection. The data from newborn urine suggests that amniotic interleukin-1ra originates from the fetus. However, amniotic fluid interleukin-1alpha and interleukin-1beta may be derived from maternal tissue, such as decidua.
研究白细胞介素-1相关物质在无宫内感染的足月分娩羊水中的作用。
收集了41例背景各异患者的羊水样本。在进行酶联免疫吸附测定（ELISA）之前，引入了一种使用氟利昂II的新型分析前处理方法，以提高细胞因子（即白细胞介素-1α、白细胞介素-1β和白细胞介素-1受体拮抗剂）的回收率。还对新生儿的尿液样本进行了检测。
正常阴道分娩样本中白细胞介素-1α、白细胞介素-1β和白细胞介素-1受体拮抗剂的浓度显著更高。在无感染的早产样本中也观察到白细胞介素-1α水平较高。与足月儿相比，早产儿尿液中产生的白细胞介素-1受体拮抗剂更多。
本研究提供了白细胞介素-1相关物质可能参与无感染迹象分娩的证据。新生儿尿液数据表明羊水白细胞介素-1受体拮抗剂源自胎儿。然而，羊水白细胞介素-1α和白细胞介素-1β可能源自母体组织，如蜕膜。
