Axelsson J, Palmblad M, Håkansson K, Håkansson P
Ion Physics Division, Angström Laboratory, Uppsala University, Sweden.
Rapid Commun Mass Spectrom. 1999;13(6):474-7. doi: 10.1002/(SICI)1097-0231(19990330)13:6<474::AID-RCM505>3.0.CO;2-1.
Electron capture dissociation of the peptide Substance P is reported for the first time, with an unmodified, commercially available Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. The fragmentation pattern is compared with that obtained with collisionally induced dissociation of the ions in the electrospray ion source, and note that electron capture dissociation gives a more easily interpreted spectrum, showing mainly C-fragments. With the exception of the proline residues, which require cleavage of two chemical bonds, we observe all C-fragmental we find the bias voltage of the electron gun not to be very critical.
首次报道了使用未修饰的市售傅里叶变换离子回旋共振（FTICR）质谱仪对P物质肽进行电子捕获解离。将该碎裂模式与在电喷雾离子源中通过离子碰撞诱导解离获得的模式进行比较，注意到电子捕获解离给出了更易于解释的谱图，主要显示C片段。除了需要断裂两个化学键的脯氨酸残基外，我们观察到所有的C片段，发现电子枪的偏置电压不是非常关键。
