Tsybin Y O, Håkansson P, Budnik B A, Haselmann K F, Kjeldsen F, Gorshkov M, Zubarev R A
Ion Physics Division, Angström Laboratory, Uppsala University, Box 534, SE-751 21 Uppsala, Sweden.
Rapid Commun Mass Spectrom. 2001;15(19):1849-54. doi: 10.1002/rcm.448.
New low-energy electron injection systems based on indirectly heated dispenser cathodes facilitate electron capture dissociation (ECD) in Fourier transform ion cyclotron resonance (FTICR) mass spectrometry. In this joint report, details are presented of the design and performance of these systems on two commercial FTICR instruments, 9.4 T Bruker BioAPEX in Uppsala and 4.7 T IonSpec Ultima in Odense. New results include obtaining meaningful one-scan MS/MS data for isolated precursor ions with millisecond irradiation times. The ECD rate improvement is not only due to the larger total electron current, but the larger emitting area as well.
基于间接加热 dispenser 阴极的新型低能电子注入系统有助于在傅里叶变换离子回旋共振（FTICR）质谱中实现电子捕获解离（ECD）。在本联合报告中，详细介绍了这些系统在两台商用 FTICR 仪器上的设计和性能，一台是位于乌普萨拉的 9.4 T 布鲁克 BioAPEX，另一台是位于欧登塞的 4.7 T 离子规格 Ultima。新成果包括在毫秒级辐照时间下，为孤立的前体离子获取有意义的单次扫描 MS/MS 数据。ECD 速率的提高不仅归因于总电子电流的增大，还得益于发射面积的增大。
