通过单链构象多态性分析及包膜糖蛋白B基因区域DNA测序鉴定人类巨细胞病毒变体——肝移植受者中的分布频率

Identification of human cytomegalovirus variants by analysis of single strand conformation polymorphism and DNA sequencing of the envelope glycoprotein B gene region-distribution frequency in liver transplant recipients.

作者信息

Binder T, Siegert W, Kruse A, Oettle H, Wilborn F, Peng R, Timm H, Neuhaus P, Schmidt C A

机构信息

Klinik für Innere Medizin m.S. Hämatologie und Onkologie, Charité, Humboldt-Universität, Berlin, Germany.

出版信息

J Virol Methods. 1999 Mar;78(1-2):153-62. doi: 10.1016/s0166-0934(98)00173-6.

DOI:10.1016/s0166-0934(98)00173-6

PMID:10204705

Abstract

Single strand conformation polymorphism analysis (SSCP) of PCR-amplified DNA and subsequent DNA sequencing of human cytomegalovirus (HCMV) glycoprotein B (gB) gene were applied to identify known HCMV strains and to detect new virus variants. 61 HCMV PCR positive patients were studied out of a cohort of 410 patients after liver transplantation (LTX). SSCP was able to distinguish between strains Davis, AD169, and Towne, and in addition could identify five new virus variants (Berlin B, C, E, F, and H). Their frequency, gB and gH types were determined. Simultaneous infections with two or three strains or variants, as well as a switch from one virus to another virus were observed during long-term follow-up. No correlation between the occurrence of certain virus strains or gB types and defined clinical manifestations of HCMV infection after LTX was drawn.

摘要

应用聚合酶链反应（PCR）扩增的人巨细胞病毒（HCMV）糖蛋白B（gB）基因的单链构象多态性分析（SSCP）及随后的DNA测序来鉴定已知的HCMV毒株并检测新的病毒变体。在410例肝移植（LTX）患者队列中，对61例HCMV PCR阳性患者进行了研究。SSCP能够区分戴维斯株、AD169株和汤氏株，此外还能鉴定出5种新的病毒变体（柏林B、C、E、F和H）。确定了它们的频率、gB和gH类型。在长期随访期间，观察到同时感染两种或三种毒株或变体，以及从一种病毒转换为另一种病毒的情况。未得出肝移植后特定病毒毒株或gB类型的出现与HCMV感染的特定临床表现之间的相关性。