Yu Zhong-sheng, Zheng Ji-yan, Wu Jia-bo
Children's Hospital Affiliate to College of Medicine, Zhejiang University, Hangzhou 310003, China.
Zhonghua Yi Xue Za Zhi. 2007 Jan 23;87(4):259-61.
To investigate the association between the clinical manifestations of infants with human cytomegalovirus (HCMV) infection and glycoprotein B (gB) genotype.
Urine samples were obtained from 107 symptomatic infants with HCMV infection confirmed by fluorescence quantitative PCR, 70 male and 37 female, aged 5 d-8 months, and 25 asymptomatic infants with HCMV infection, 16 male and 9 female, aged 21 d-7 months. A fragment of glycoprotein B gene was amplified by nested PCR (nPCR). HCMV gB genotyping was carried out by restriction fragment length polymorphism (RFLP), and some of the amplified DNA fragments were verified by DNA sequencing.
Of the HCMV specimens from 107 infants, 53 were typed as gB group I, 20 as gB II, 18 as gB III, 7 as gB I and gB II, 5 as gB I and gB III, and 4 as gB II and gB III, and gB IV was not found. The HCMV gB genotype from 53 infants with hepatic function damage showed that 36 were classified as gB I, 5 as gB II, 7 as gB III, 3 as gB I and gB II, and 2 as gB I and gB III. The gB I genotype was more common among the infants with hepatic function damage (P < 0.05). The distribution of gB genotype in the asymptomatic infants was as follow: gB I, 10/25; gB II, 6/25; gB III, 8/25; and gB I and gB II, 1/25. The homology of PCR products of HCMV gB in 24 strains amplified compared with the sequences of prototype strains in GenBank was from 97.06% to 99.64%.
RFLP analysis of HCMV gB genotype is definite and reliable. The gB I genotype is more common among the infants with hepatic function damage.
探讨人巨细胞病毒（HCMV）感染婴儿的临床表现与糖蛋白B（gB）基因型之间的关联。
收集107例经荧光定量PCR确诊为HCMV感染的有症状婴儿的尿液样本，其中男70例，女37例，年龄5天至8个月；以及25例HCMV感染的无症状婴儿的尿液样本，其中男16例，女9例，年龄21天至7个月。采用巢式PCR（nPCR）扩增糖蛋白B基因片段。通过限制性片段长度多态性（RFLP）进行HCMV gB基因分型，部分扩增的DNA片段通过DNA测序进行验证。
107例婴儿的HCMV标本中，53例为gB I组，20例为gB II组，18例为gB III组，7例为gB I和gB II组，5例为gB I和gB III组，4例为gB II和gB III组，未发现gB IV型。53例肝功能损害婴儿的HCMV gB基因型显示，36例为gB I型，5例为gB II型，7例为gB III型，3例为gB I和gB II型，2例为gB I和gB III型。gB I基因型在肝功能损害婴儿中更为常见（P<0.05）。无症状婴儿中gB基因型分布如下：gB I型，10/25；gB II型，6/25；gB III型，8/25；gB I和gB II型，1/25。24株扩增的HCMV gB的PCR产物与GenBank中原型株序列的同源性为97.06%至99.64%。
HCMV gB基因型的RFLP分析明确可靠。gB I基因型在肝功能损害婴儿中更为常见。
