Mittendorfer B, Jeschke M G, Wolf S E, Sidossis L S
Department of Surgery, The University of Texas Medical Branch, Galveston, TX 77550, USA.
Clin Nutr. 1998 Dec;17(6):293-9. doi: 10.1016/s0261-5614(98)80322-1.
To investigate the effects of diet composition and starvation on hepatic steatosis and mortality after severe burn injury in rats.
Experiment 1: rats received either normal chow (55 cent of energy carbohydrates, 14 cent fat, 31 cent protein), a high-fat (40 cent carbohydrates, 40 cent fat, 20 cent protein), or a high-carbohydrate diet (81 cent carbohydrates, 4 cent fat, 15 cent protein) ad libitum for 6 days. Another three groups received these diets ad libitum for 6 days after 48|h starvation. Experiment 2: mortality after 60 cent total body surface area scald burn was determined in a control group of rats and a group with nutritionally induced hepatic steatosis. Hepatic steatosis was induced by feeding the rats a high-fat diet (40 cent carbohydrates, 4 cent fat, 15 cent protein) ad libitum for 6 days.
Without starvation, liver triglyceride content (mg/g liver) increased in response to the high-fat diet (25.6'6.9) compared to normal chow (9.4'3.8; P < 0. 05); the high-carbohydrate diet had no influence on liver triglyceride content (12.4'3.7). Refeeding after starvation resulted in elevated (P < 0.05) liver triglyceride content in the high-fat (18.8'8.3) and the high-carbohydrate group (28.7'14.4 vs control 6. 7'3.7). Liver triglyceride content correlated (R2=0.72; P < 0.05) to non-protein energy intake but not to total energy intake. Burn caused 33 cent mortality in the hepatic steatosis group and no deaths in the control group (P < 0.05).
Diet composition and preceding starvation independently manipulate hepatic fat content in rats. Hepatic steatosis increases mortality after burn injury. Thus, nutritional interventions to reduce hepatic fat accumulation may be beneficial.
研究饮食组成和饥饿对大鼠严重烧伤后肝脂肪变性及死亡率的影响。
实验1：大鼠自由摄取普通饲料（能量的55%为碳水化合物，14%为脂肪，31%为蛋白质）、高脂肪饲料（40%碳水化合物，40%脂肪，20%蛋白质）或高碳水化合物饲料（81%碳水化合物，4%脂肪，15%蛋白质）6天。另外三组在饥饿48小时后自由摄取这些饲料6天。实验2：测定60%体表面积烫伤烧伤大鼠对照组和营养性肝脂肪变性组的死亡率。通过让大鼠自由摄取高脂肪饲料（40%碳水化合物，4%脂肪，15%蛋白质）6天诱导肝脂肪变性。
在无饥饿状态下，与普通饲料（9.4±3.8；P<0.05）相比，高脂肪饲料组肝脏甘油三酯含量（mg/g肝脏）升高（25.6±6.9）；高碳水化合物饲料对肝脏甘油三酯含量无影响（12.4±3.7）。饥饿后再喂食导致高脂肪组（18.8±8.3）和高碳水化合物组（28.7±14.4，对照组为6.7±3.7）肝脏甘油三酯含量升高（P<0.05）。肝脏甘油三酯含量与非蛋白质能量摄入相关（R2=0.72；P<0.05），但与总能量摄入无关。烧伤导致肝脂肪变性组死亡率为33%，对照组无死亡（P<0.05）。
饮食组成和先前的饥饿状态独立影响大鼠肝脏脂肪含量。肝脂肪变性增加烧伤后死亡率。因此，减少肝脏脂肪堆积的营养干预可能有益。
