Nomura F, Ohnishi K, Suzuki T, Tanaka H, Tsunoda T, Tanabe Y, Iida S, Okuda K
First Department of Medicine, Chiba University School of Medicine, Chiba University Hospital, Japan.
Int J Obes. 1987;11(6):603-8.
In order to test whether or not overeating of a nutritionally adequate diet with reasonable fat content could result in significant fat accumulation in the liver, male Sprague-Dawley rats were provided with free access to either a nutritionally adequate liquid diet with 35 per cent of calories as fat or a regular diet (controls) for 3 months. After the feeding period, body weight, Lee index, and epididymal adipose tissue weight, were significantly greater in rats fed with the liquid diet than in the controls. Liver weight, hepatic triglyceride levels were also greater in the liquid diet group. Histologically, remarkable fatty infiltration was observed predominantly in periportal areas in rats fed with the liquid diet ad libitum for 3 months. Compared to a large body of the literature concerning diet-induced obesity in experimental animals, information on animal models of fatty liver by dietary manipulations is insufficient. The results of this study clearly indicate that the overeating of a nutritionally adequate diet with reasonable fat content could result in remarkable fat accumulation in the liver in rats.
为了测试食用营养充足且脂肪含量合理的饮食过量是否会导致肝脏中显著的脂肪堆积，给雄性斯普拉格-道利大鼠提供了两种饮食选择，为期3个月：一种是营养充足的液体饮食，其中35%的热量来自脂肪；另一种是常规饮食（对照组）。在喂食期结束后，喂食液体饮食的大鼠的体重、李氏指数和附睾脂肪组织重量显著高于对照组。液体饮食组的肝脏重量和肝甘油三酯水平也更高。组织学上，在自由采食液体饮食3个月的大鼠中，主要在门静脉周围区域观察到明显的脂肪浸润。与大量关于实验动物饮食诱导肥胖的文献相比，通过饮食操作建立脂肪肝动物模型的信息不足。本研究结果清楚地表明，食用营养充足且脂肪含量合理的饮食过量会导致大鼠肝脏中显著的脂肪堆积。
