Iarmol'chuk S G
Klin Lab Diagn. 1998 Nov(11):39-41.
Urine of diabetics without concomitant diseases with a certain concentration of glucose was collected for preparing reference material. Water saturated with phenol (872 mmole/liter) was added to urine pool during intensive stirring on a magnetic stirrer at 20 degrees C, so that the final concentration of phenol were 50 mmole/liter. Glucose content in the resultant reference material remained stable for 130 days at 18-20 degrees C. For glucose measurements, 2 ml reference material and 0.5 ml 2.5 M sodium oxide hydrate are put in centrifuge tubes, stirred, put on boiling water bath for 2 min, cooled, and colorimetried in cuvettes with 1-mm optic route. The content of monosaccharide is calculated using the proportion rule in comparison with the values for 139 mM reference glucose solution in control urine.
收集无伴发疾病且具有一定葡萄糖浓度的糖尿病患者尿液以制备参考物质。在20℃下于磁力搅拌器上剧烈搅拌期间，向尿液池中加入饱和苯酚水溶液（872毫摩尔/升），以使苯酚的最终浓度为50毫摩尔/升。所得参考物质中的葡萄糖含量在18 - 20℃下130天内保持稳定。进行葡萄糖测量时，将2毫升参考物质和0.5毫升2.5M氢氧化钠水合物放入离心管中，搅拌，置于沸水浴中2分钟，冷却，然后在光程为1毫米的比色皿中进行比色测定。与对照尿液中139mM参考葡萄糖溶液的值相比，使用比例规则计算单糖含量。
