Liu Y, Li Z, Bakhiet M
Division of Neurology, Karolinska Institute, Huddinge University Hospital, Sweden.
Trop Med Int Health. 1999 Feb;4(2):85-92. doi: 10.1046/j.1365-3156.1999.00364.x.
We examined the induction of 4 chemokines during early experimental African trypanosomiasis using in situ hybridization and immunocytochemistry. mRNA expression and protein production of Rantes, MCP-1, MIP-1a and MIP-2 were studied in splenocytes obtained at 0 h, 4 h and 12 h post-infection. Splenic denervation was performed to study the role of the central nervous system in early infection. The mRNA for Rantes increased at 4 h and declined at 12 h, but the protein level was high at both time-points. MCP-1 and MIP-la had elevated mRNA and protein levels at 12 h post-infection. MIP-2 mRNA was high at both 4 h and 12 h, but the protein level was only increased at 12 h. Splenic denervation, but not sham operation, suppressed these responses. The upregulation of these chemokines during very early infection suggests a chemokine role in the developing immunopathology The sympathetic nervous system may, however, participate in modulation of such early immune responses.
我们使用原位杂交和免疫细胞化学方法，研究了早期实验性非洲锥虫病期间4种趋化因子的诱导情况。在感染后0小时、4小时和12小时获取的脾细胞中，研究了调节激活正常T细胞表达和分泌的趋化因子（RANTES）、单核细胞趋化蛋白-1（MCP-1）、巨噬细胞炎性蛋白-1α（MIP-1α）和巨噬细胞炎性蛋白-2（MIP-2）的mRNA表达和蛋白产生情况。进行脾去神经支配以研究中枢神经系统在早期感染中的作用。RANTES的mRNA在4小时时增加，在12小时时下降，但在两个时间点蛋白水平都很高。感染后12小时，MCP-1和MIP-1α的mRNA和蛋白水平升高。MIP-2的mRNA在4小时和12小时时均较高，但蛋白水平仅在12小时时增加。脾去神经支配而非假手术抑制了这些反应。在极早期感染期间这些趋化因子的上调表明趋化因子在发展中的免疫病理学中起作用。然而，交感神经系统可能参与调节这种早期免疫反应。
