Krizamová H, Koppel Z, Halasa M, Beseda M, Horváth J
Vet Med (Praha). 1978 Oct;23(10):633-40.
Five hundred and thirty-six samples of honeycombs were examined in a laboratory in the years 1971-1974. In all the samples clinically determined as the foul brood, B. alvei was isolated as a pure culture, and enterococci, or both microorganisms were isolated in mixed form. Twenty-five strains of the isolated streptococci were analyzed microbiologically and biochemically; on the basis of their culture and biochemical characteristics five strains were designated as Streptococcus faecalis, 14 strains as Streptococcus faecalis var. liquefaciens, five strains as Streptococcus faecium and one strain as Streptococcus durans. After checking the used taxonomic key of the culture and biochemical classification of B. alvei it may be stated that the culture and biochemical characteristics are stable. The strains of B. alvei (very dried strains), which persisted in the dried slant meat-peptone agar, were viable under the laboratory conditions, which proves the high resistance of the spores to the environment.
1971年至1974年期间,在实验室对536个蜂巢样本进行了检测。在所有临床上判定为美洲幼虫腐臭病的样本中,均分离出纯培养的蜂房蜜蜂球菌,或者分离出肠球菌,或者两种微生物以混合形式被分离出来。对分离出的25株链球菌进行了微生物学和生化分析;根据它们的培养和生化特性,5株被定为粪肠球菌,14株为液化粪肠球菌变种,5株为屎肠球菌,1株为耐久肠球菌。在检查了所使用的蜂房蜜蜂球菌培养和生化分类的分类检索表后可以表明,培养和生化特性是稳定的。在干燥的斜面肉蛋白胨琼脂上存活的蜂房蜜蜂球菌菌株(非常干燥的菌株)在实验室条件下仍具活力,这证明了孢子对环境具有高度抗性。