Black S J, Wang Q, Makadzange T, Li Y L, Van Praagh A, Loomis M, Seed J R
Department of Veterinary and Animal Sciences, Paige Laboratory, University of Massachusetts, Amherst 01003, USA.
J Parasitol. 1999 Feb;85(1):48-53.
Constitutive anti-Trypanosoma brucei subsp. brucei S 427 clone 1 and 22 activities were evaluated in sera from 22 species of nonprimate mammals. The sera fell into 5 categories. Sera from Cape buffalo, giraffe, and greater kudu showed a concentration-dependent inhibition of replication of the 2 clones of organisms, which was dependent on the presence of xanthine oxidase. Sera from warthog and springbok also severely limited trypanosome replication but lacked xanthine oxidase. Their antitrypanosome activity was inactivated by heating at 56 C for 30 min but not affected by absorbing with trypanosomes at 4 C. Sera from lion and leopard showed a concentration-dependent inhibition of the growth of T. brucei S427 clone 1 organisms, but not clone 22 organisms. These sera lacked xanthine oxidase. Their anti-T. brucei S 427 clone 1 activity was inactivated by heating at 56 C for 30 min but not removed by absorbing with trypanosomes. Serum from Grant's gazelle prevented replication of both T. brucei clones, lacked xanthine oxidase, and was not affected by heating at 56 C. Sera from waterbuck, Thompson's gazelle, sitatunga, Cape hartebeeste, gerenuk, Grant's zebra, cow, several cat, cougar, bobcat, and domestic cat were fully supportive of trypanosome replication irrespective of concentration tested up to a maximum of 48% v/v in culture medium. Sera from different individuals of the same mammal species had similar effects on trypanosomes, and samples collected from the same individual at different times also had similar activities indicating species-specific stable expression, or lack thereof, of constitutive serum antitrypanosome components.
对来自22种非灵长类哺乳动物的血清中组成型抗布氏锥虫亚种布氏锥虫S 427克隆1和22的活性进行了评估。这些血清分为5类。非洲水牛、长颈鹿和大林羚的血清对这2个克隆的生物体复制表现出浓度依赖性抑制，这取决于黄嘌呤氧化酶的存在。疣猪和跳羚的血清也严重限制了锥虫的复制，但缺乏黄嘌呤氧化酶。它们的抗锥虫活性在56℃加热30分钟后失活，但在4℃用锥虫吸附不受影响。狮子和豹的血清对布氏锥虫S427克隆1生物体的生长表现出浓度依赖性抑制，但对克隆22生物体无抑制作用。这些血清缺乏黄嘌呤氧化酶。它们的抗布氏锥虫S 427克隆1活性在56℃加热30分钟后失活，但不能通过用锥虫吸附去除。格兰特瞪羚的血清可阻止两种布氏锥虫克隆的复制，缺乏黄嘌呤氧化酶，且不受56℃加热的影响。水羚、汤氏瞪羚、水獭羚、南非大羚羊、长颈羚、格兰特斑马、牛、几种猫科动物、美洲狮、短尾猫和家猫的血清在培养基中浓度高达48% v/v时，无论测试浓度如何，都完全支持锥虫的复制。同一哺乳动物物种不同个体的血清对锥虫的作用相似，在不同时间从同一个体采集的样本也具有相似的活性，表明组成型血清抗锥虫成分具有物种特异性的稳定表达或缺乏这种表达。
