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自然感染北极狐体内本地毛形线虫的耐冻性、形态学及随机扩增多态性DNA聚合酶链式反应鉴定

Freeze tolerance, morphology, and RAPD-PCR identification of Trichinella nativa in naturally infected arctic foxes.

作者信息

Kapel C M, Pozio E, Sacchi L, Prestrud P

机构信息

Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.

出版信息

J Parasitol. 1999 Feb;85(1):144-7.

PMID:10207384
Abstract

Arctic foxes (Alopex lagopus) were collected from Greenland and Svalbard (N = 319). Twenty-four were infected with Trichinella (7.5%). Molecular analysis (random-amplified polymorphic DNA polymerase chain reaction) confirmed that all animals were infected with Trichinella nativa. Motile larvae were found in muscle tissue from all foxes after carcasses had been frozen for 1 yr at -18 C. Infective larvae were found in 2 foxes after a total of 4 yr storage at -18 C, which is longer than any previous observations. Morphological examination of the cysts showed large nurse cells and significant deposition of collagen and connective tissue. It is suggested that, within the geographical distribution of T. nativa, the more freeze-resistant isolates are found at higher latitudes.

摘要

北极狐(Alopex lagopus)取自格陵兰岛和斯瓦尔巴群岛(N = 319)。其中24只感染了旋毛虫(7.5%)。分子分析(随机扩增多态性DNA聚合酶链反应)证实所有动物均感染了本地旋毛虫。在尸体于-18℃冷冻1年后,所有狐狸的肌肉组织中均发现了活动幼虫。在-18℃总共储存4年后,在2只狐狸中发现了感染性幼虫,这比以往任何观察结果的时间都长。对囊肿的形态学检查显示有大的滋养细胞以及大量胶原蛋白和结缔组织沉积。有人提出,在本地旋毛虫的地理分布范围内,在较高纬度地区发现的分离株抗冻性更强。

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