Walter G L
MPI Research, Mattawan, Michigan, USA.
Toxicol Pathol. 1999 Mar-Apr;27(2):217-25. doi: 10.1177/019262339902700208.
Blood samples from adult male and female Charles River Crl:CD (SD) BR rats were collected at weekly intervals for 4 wk to evaluate the effects of inhalation of an anesthetic dose of carbon dioxide (CO2) or of a carbon dioxide-oxygen mixture (CO2/O2) on hematology, coagulation, and serum biochemistry values. During the first 3 wk of the study, rats were assigned to 1 of 3 groups and were bled from the orbital sinus once weekly. Prior to the blood collection, rats in group 1 were exposed to room air only, rats in group 2 received CO2/O2 (approximately 66%:34% CO2:O2) by inhalation, and rats in group 3 received 100% CO2 by inhalation. In the rats exposed to CO2/O2 or CO2, leukocyte counts, lymphocyte counts, and glucose values were higher, and aspartate aminotransferase, creatine kinase, and calcium values were lower compared with those of rats exposed to room air only. Rats exposed to 100% CO2 had slightly (but statistically significant) lower mean corpuscular hemoglobin concentration when compared with rats exposed only to room air. During week 4, all rats were reassigned to 1 of 2 groups and were bled terminally via closed cardiac puncture following exposure to either CO2/O2 or CO2. Increased lymphocyte counts (males only) and glucose and chloride concentrations were noted for rats exposed to CO2/O2 compared with those exposed to CO2. These alterations reiterate the importance of comparing clinical pathology values to those of concurrent control groups that have experienced blood collection under identical conditions in order to avoid potential errors in the interpretation of data.
每周采集成年雄性和雌性Charles River Crl:CD(SD)BR大鼠的血样，持续4周，以评估吸入麻醉剂量的二氧化碳(CO2)或二氧化碳 - 氧气混合物(CO2/O2)对血液学、凝血和血清生化值的影响。在研究的前3周，将大鼠分为3组中的1组，每周从眶窦取血一次。采血前，第1组大鼠仅暴露于室内空气中，第2组大鼠通过吸入接受CO2/O2(约66%:34% CO2:O2)，第3组大鼠通过吸入接受100% CO2。与仅暴露于室内空气的大鼠相比，暴露于CO2/O2或CO2的大鼠白细胞计数、淋巴细胞计数和血糖值较高，而天冬氨酸转氨酶、肌酸激酶和钙值较低。与仅暴露于室内空气的大鼠相比，暴露于100% CO2的大鼠平均红细胞血红蛋白浓度略低(但具有统计学意义)。在第4周，所有大鼠重新分配到2组中的1组，在暴露于CO2/O2或CO2后通过封闭心脏穿刺进行终末采血。与暴露于CO2的大鼠相比，暴露于CO2/O2的大鼠淋巴细胞计数增加(仅雄性)，葡萄糖和氯化物浓度升高。这些改变再次强调了将临床病理学值与在相同条件下进行采血的同期对照组的值进行比较的重要性，以避免数据解释中的潜在错误。
