Division of Comparative Medicine, Washington University in St Louis, St Louis, Missouri;, Email:
National Institutes of Health, NIMH, Bethesda, Maryland.
J Am Assoc Lab Anim Sci. 2020 May 1;59(3):242-253. doi: 10.30802/AALAS-JAALAS-19-000084. Epub 2020 Mar 5.
The optimal choice of euthanasia method for laboratory rodents depends on a number of factors, including the scientific goals of the study, the need to minimize animal pain and/or distress, applicable guidelines and laws, the training and proficiency of personnel, and the safety and emotional needs of the personnel performing the euthanasia. This manuscript aims to provide guidance to researchers so they may select the method of euthanasia that results in minimal experimental confounds, such as the creation of artifact and alteration of tissues and analytes. Specific situations addressed include euthanasia of large numbers of rodents and euthanasia of neonates. Recent literature supports the notion of significant strain-dependent differences in response to euthanasia methods such as CO₂ inhalation. To assist researchers in selecting a strain-appropriate method of euthanasia, the authors present a summary of methodologies for assessing the effectiveness of euthanasia techniques, including elements and parameters for a scoring rubric to assess them.
实验室啮齿动物安乐死方法的最佳选择取决于许多因素，包括研究的科学目标、最大限度减少动物疼痛和/或痛苦的需求、适用的准则和法律、人员的培训和熟练程度，以及执行安乐死人员的安全和情感需求。本文旨在为研究人员提供指导，以便他们选择可将实验干扰降至最低的安乐死方法，例如避免产生伪迹以及改变组织和分析物。具体情况包括大量啮齿动物的安乐死和新生仔鼠的安乐死。最近的文献支持这样一种观点，即不同品系的啮齿动物对二氧化碳吸入等安乐死方法的反应存在显著的依赖性差异。为了帮助研究人员选择适合品系的安乐死方法，作者总结了评估安乐死技术效果的方法学，包括评估安乐死技术的评分标准的要素和参数。
