一种用于鉴别淋病奈瑟菌产β-内酰胺酶质粒的聚合酶链反应检测方法。

A PCR assay for discriminating Neisseria gonorrhoeaebeta-lactamase-producing plasmids.

作者信息

Dillon J R, Li H, Yeung K, Aman T A

机构信息

Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada.

出版信息

Mol Cell Probes. 1999 Apr;13(2):89-92. doi: 10.1006/mcpr.1998.0216.

DOI:10.1006/mcpr.1998.0216

PMID:10208798

Abstract

Oligonucleotide primers were developed for use in polymerase chain reaction (PCR) assays to differentiate three related, epidemic beta-lactamase-producing plasmids of Neisseria gonorrhoeae-the Asia-(7426 bp), Africa-(5599 bp) and Toronto-(5154 bp) type plasmids. One-hundred and two N. gonorrhoeae isolates with different plasmid profiles were tested-16 isolates carried the Asia plasmid, 41 isolates contained the Africa plasmid, 16 isolates contained the Toronto plasmid and 29 isolates contained no beta-lactamase-producing plasmids. Most (101/102) isolates also carried the gonococcal cryptic plasmid, while 27/102 and 44/102 isolates carried either the transfer plasmid or the tet M-containing plasmids, respectively. The assay was 100% sensitive and specific for identifying the correct plasmid type. This assay is useful for rapidly detecting the presence of gonococcal beta-lactamase-producing plasmids in clinical samples and discriminating them for epidemiological typing.

摘要

开发了寡核苷酸引物，用于聚合酶链反应（PCR）分析，以区分淋病奈瑟菌的三种相关的、流行的产β-内酰胺酶质粒——亚洲型（7426 bp）、非洲型（5599 bp）和多伦多型（5154 bp）质粒。对102株具有不同质粒图谱的淋病奈瑟菌分离株进行了检测——16株携带亚洲质粒，41株含有非洲质粒，16株含有多伦多质粒，29株不含有产β-内酰胺酶质粒。大多数（101/102）分离株还携带淋球菌隐蔽质粒，而27/102和44/102分离株分别携带转移质粒或含tet M质粒。该分析对于鉴定正确的质粒类型具有100%的敏感性和特异性。该分析可用于快速检测临床样本中淋病奈瑟菌产β-内酰胺酶质粒的存在，并对其进行流行病学分型鉴别。