Mansy M S, Fadl A A, Ashour M S, Khan M I
Department of Pathobiology, University of Connecticut, Storrs, CT 06269-3089, USA.
Mol Cell Probes. 1999 Apr;13(2):133-40. doi: 10.1006/mcpr.1999.0226.
A Proteus mirabilis-specific polymerase chain reaction (PCR) was developed and standardized. The origin of the primers was a recombinant clone that contained P. mirabilis-specific Hind III fragment DNA of 3.5-kilobase pairs. Based on the sequence data of P. mirabilis recombinant clone, two primers designated MMKAP 1 and MMKAP 2 were synthesized for use in the PCR. A P. mirabilis-specific 3.5-kb pair DNA product was amplified by the primers from 18 strains of P. mirabilis, but not from other Protease species and bacteria. The minimum amount of target DNA detected by P. mirabilis PCR was 10 fg using ethidium bromide/ultraviolet exposure of gels or Southern blot hybridization with a P. mirabilis recombinant DNA probe.
开发并标准化了一种奇异变形杆菌特异性聚合酶链反应（PCR）。引物来源于一个重组克隆，该克隆包含3.5千碱基对的奇异变形杆菌特异性Hind III片段DNA。根据奇异变形杆菌重组克隆的序列数据，合成了两条引物，命名为MMKAP 1和MMKAP 2，用于PCR。引物从18株奇异变形杆菌中扩增出一条3.5千碱基对的奇异变形杆菌特异性DNA产物，但其他变形杆菌属物种和细菌未扩增出该产物。使用溴化乙锭/凝胶紫外线曝光或与奇异变形杆菌重组DNA探针进行Southern印迹杂交，奇异变形杆菌PCR检测到的目标DNA的最小量为10 fg。
