使用基于重复序列的聚合酶链反应（rep-PCR）对变形杆菌属进行基因组指纹分析。
Genomic fingerprinting of Proteus species using repetitive sequence based PCR (rep-PCR).
作者信息
Serwecińska L, Cieślikowski T, Pytlos M, Jaworski A, Kaca W
机构信息
Institute of Microbiology and Immunology, University of Lódź, Poland.
出版信息
Acta Microbiol Pol. 1998;47(3):313-9.
Three Proteus species P. vulgaris, P. mirabilis and P. penneri have been characterized by repetitive sequence-based PCR. Four families of repetitive sequence based primers REP, ERIC, BOXA1R and BOXA2R, give specific patterns for each Proteus species. Species differentiation was best afforded using BOXA2R for detection of P. mirabilis, either REP-Dt or BOXA1R primers for detection of P. penneri and ERIC primer pair for P. vulgaris.
三种变形杆菌属细菌，即普通变形杆菌、奇异变形杆菌和彭氏变形杆菌，已通过基于重复序列的聚合酶链反应进行了特征分析。基于重复序列的四类引物，即REP、ERIC、BOXA1R和BOXA2R，可为每种变形杆菌属细菌产生特定的图谱。使用BOXA2R检测奇异变形杆菌、使用REP-Dt或BOXA1R引物检测彭氏变形杆菌以及使用ERIC引物对检测普通变形杆菌，能最好地实现菌种鉴别。
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