Erhard M, von Döhren H, Jungblut P R
Institut für Biochemie und Molekulare Biologie, Technische Universität Berlin, Germany.
Rapid Commun Mass Spectrom. 1999;13(5):337-43. doi: 10.1002/(SICI)1097-0231(19990315)13:5<337::AID-RCM488>3.0.CO;2-Q.
Toxic water blooms from cyanobacteria in lakes and rivers are a worldwide phenomenon. A new technique is presented for the rapid detection of toxic and nontoxic blooms. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was employed to identify mainly peptide metabolites (microcystins, anabaenopeptins, cyanopeptolins, microviridins, microginins and aeruginosins) from microgram quantities of prepared cells within minutes. The spectra show the presence of peptides in strains or water blooms simultaneously. A new compound has been identified using the post source decay (PSD) and collision induced dissociation (CID) mode. This new compound has been defined as anabaenoeptin G. The potential of the method for screening of various secondary metabolite producers for defined products including antibiotics is discussed.
湖泊和河流中蓝藻产生的有毒水华是一种全球现象。本文介绍了一种快速检测有毒和无毒水华的新技术。采用基质辅助激光解吸/电离飞行时间质谱(MALDI-TOFMS)在数分钟内从微克量的制备细胞中主要鉴定肽代谢产物(微囊藻毒素、鱼腥藻肽、蓝藻肽、微绿藻素、微吉宁和铜绿菌素)。光谱同时显示了菌株或水华中肽的存在。利用源后衰变(PSD)和碰撞诱导解离(CID)模式鉴定出一种新化合物。这种新化合物被定义为鱼腥藻肽G。本文还讨论了该方法用于筛选包括抗生素在内的特定产品的各种次生代谢产物生产者的潜力。