Erhard M, von Döhren H, Jungblut P
Institute of Biochemistry and Molecular Biology, Technical University Berlin, Germany.
Nat Biotechnol. 1997 Sep;15(9):906-9. doi: 10.1038/nbt0997-906.
Toxic cyanobacterial blooms are a threat because of secondary metabolite production. We used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to identify intact microorganisms. Microgram quantities of prepared cells, including solvent (acetonitrile and ethanol) and alpha-cyano-4-hydroxycinnamic acid matrix, display spectra showing predominantly the secondary metabolites including known microcystins, micropeptin, and anabaenopeptolin. A new cyclic anabaenopeptolin has been identified using the Post-Source-Decay mode. Strains of various origins can easily be typed according to their cyclic peptide production, and toxic and nontoxic algal blooms can be differentiated within minutes.
有毒蓝藻水华因产生次生代谢产物而构成威胁。我们使用基质辅助激光解吸/电离飞行时间质谱法来鉴定完整的微生物。微克级量的制备细胞，包括溶剂（乙腈和乙醇）和α-氰基-4-羟基肉桂酸基质，所显示的光谱主要呈现次生代谢产物，包括已知的微囊藻毒素、微肽菌素和节球藻肽。已使用源后衰变模式鉴定出一种新的环状节球藻肽。各种来源的菌株可根据其环状肽的产生轻松分型，并且可在数分钟内区分有毒和无毒藻华。
