Cavic B A, Thompson M
Department of Chemistry, University of Toronto, ON, Canada.
Analyst. 1998 Oct;123(10):2191-6. doi: 10.1039/a804225j.
Surfaces of the two organosiloxanes, polymercaptopropylmethylsiloxane and octaphenylcyclotetrasiloxane, were prepared on the gold electrodes of thickness-shear mode acoustic wave sensors. Compounds containing the siloxane bond are important in the fabrication of medical implants. The flow-through adsorption of the proteins: human serum albumin, alpha-chymotripsinogen A, cytochrome c, fibrinogen, hemoglobin, immunoglobulin G and apo-transferrin to the two siloxane surfaces and a gold electrode were detected by acoustic network analysis. With the exception of minor wash-off by buffer flow, the adsorption of all proteins to the three surfaces is irreversible. Differences observed for the magnitudes of adsorption for the various cases are ascribed to the role played by molecular interactions at the liquid/solid interface. The results confirm that changes in series resonant frequencies caused by macromolecular adsorption differ significantly from the widely accepted "mass based" model usually employed to characterize the response of this type of acoustic wave device.
在厚度剪切模式声波传感器的金电极上制备了两种有机硅氧烷——聚巯基丙基甲基硅氧烷和八苯基环四硅氧烷的表面。含硅氧烷键的化合物在医用植入物制造中很重要。通过声学网络分析检测了蛋白质（人血清白蛋白、α-胰凝乳蛋白酶原A、细胞色素c、纤维蛋白原、血红蛋白、免疫球蛋白G和脱铁转铁蛋白）在两种硅氧烷表面和金电极上的流通吸附情况。除了缓冲液流动造成的少量洗脱外，所有蛋白质在这三种表面上的吸附都是不可逆的。不同情况下观察到的吸附量差异归因于液/固界面处分子相互作用所起的作用。结果证实，由大分子吸附引起的串联谐振频率变化与通常用于表征此类声波装置响应的广泛接受的“基于质量”模型有显著差异。
