Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON M5S 3H6, Canada.
Sensors (Basel). 2020 May 5;20(9):2628. doi: 10.3390/s20092628.
A thickness-shear mode acoustic wave biosensor operated within a flow-through system was used to examine the response of mouse retinal tissue to radiation. Control experiments conducted with respect to exposure of the bare gold electrodes of the device under various conditions of light intensity and bathing solution yielded reversible changes in resonant frequency (Fs) and motional resistance (Rm). The magnitude of transient changes was proportional to light intensity, but independent of solution type. These alterations in acoustic parameters were ascribed to acoustic coupling phenomena at the electrode-to-liquid interface. Pre-differentiated retina from mouse samples deposited on the thickness shear mode (TSM) electrode exposed to a high light intensity condition also exhibited reversible changes in both Fs and Rm, compared to control experiments involving a coating used to attach the tissue to the electrode. In this case, the radiation-instigated reversible responses for both acoustic parameters exhibited a reduction in magnitude. The changes are ascribed to the alteration in viscoelasticity of the retinal matrix on the TSM electrode surface. The precise biophysical mechanism responsible for the changes in Fs and Rm remains a challenge, given the complex make up of retinal tissue.
一种在流动系统中工作的厚度剪切模式声波生物传感器被用于研究小鼠视网膜组织对辐射的反应。在不同的光强和浴液条件下对设备的裸露金电极进行的对照实验产生了共振频率（Fs）和运动电阻（Rm）的可逆变化。瞬态变化的幅度与光强成正比，但与溶液类型无关。这些声学参数的变化归因于电极-液体界面的声耦合现象。与涉及将组织附着到电极的涂层的对照实验相比，在高光强条件下暴露于厚度剪切模式（TSM）电极的来自小鼠样本的预分化视网膜也表现出 Fs 和 Rm 的可逆变化。在这种情况下，两个声学参数的辐射诱导可逆响应的幅度都减小了。这些变化归因于 TSM 电极表面视网膜基质粘弹性的改变。鉴于视网膜组织的复杂构成，对于 Fs 和 Rm 变化的确切生物物理机制仍然是一个挑战。
