Cloak O M, Duffy G, Sheridan J J, McDowell D A, Blair I S
National Food Centre, Castleknock, Dublin, Ireland.
J Appl Microbiol. 1999 Apr;86(4):583-90. doi: 10.1046/j.1365-2672.1999.00698.x.
A rapid method based on bacterial adhesion was developed for the detection of Salmonella in an enriched meat system. Minced beef samples inoculated with Salm. enteritidis (10 cfu g-1) were incubated overnight (18 h) at 37 degrees C in buffered peptone water. Salmonella enteritidis cells were isolated from the enriched meat sample by surface adhesion onto a polycarbonate membrane attached to a glass slide. The organisms attached to this polycarbonate membrane were subsequently visualized using immunofluorescent microscopy. The technique had a detection level of log10 3.5 Salmonella ml-1. The surface adhesion immunofluorescent technique correlated well with Salmonella plate counts (r2 = 0.99). Application of the rapid method to retail beef and poultry samples (n = 100) confirmed the correlation between this technique and traditional microbiological procedures. Thirty-one retail samples were reported positive for Salmonella species. No false positives or negatives were recorded for the rapid method.
开发了一种基于细菌黏附的快速方法，用于检测富集肉体系中的沙门氏菌。接种肠炎沙门氏菌（10 cfu g-1）的碎牛肉样品在缓冲蛋白胨水中于37℃孵育过夜（18小时）。通过表面黏附到附着在载玻片上的聚碳酸酯膜上，从富集的肉样中分离出肠炎沙门氏菌细胞。随后使用免疫荧光显微镜观察附着在该聚碳酸酯膜上的微生物。该技术的检测水平为每毫升10的3.5次方个沙门氏菌。表面黏附免疫荧光技术与沙门氏菌平板计数相关性良好（r2 = 0.99）。将该快速方法应用于零售牛肉和家禽样品（n = 100），证实了该技术与传统微生物学程序之间的相关性。31个零售样品报告沙门氏菌属呈阳性。该快速方法未记录到假阳性或假阴性结果。
