Sheridan J J, Logue C M, McDowell D A, Blair I S, Hegarty T, Toivanen P
National Food Centre, Castleknock, Dublin, Ireland.
J Appl Microbiol. 1998 Oct;85(4):737-45. doi: 10.1111/j.1365-2672.1998.00584.x.
This study examined the attachment kinetics of Yersinia enterocolitica serotype O:3 to determine the optimum conditions for its isolation from meat enrichment systems using a novel surface adhesion technique. Minced beef was inoculated with Y. enterocolitica at an initial level of 10 cfu g-1 and incubated at 25 degrees C in an enrichment broth. Yersinia was recovered from enriched samples on polycarbonate membranes by surface adhesion and enumerated using immunofluorescence microscopy. The surface adhesion immunofluorescence technique (SAIF) had a minimum detection limit of approximately 4.0-4.5 log10 cfu ml-1 and provided good correlation between the estimation of the numbers of Yersinia in the enrichment broth derived from plate counts on Yersinia Selective agar (CIN) and those determined by SAIF (r2 = 0.94; rsd = +/- 0.21). A derived regression equation of the SAIF count vs plate counts was used to predict Y. enterocolitica numbers in spiked meat samples stored at 0 degree C for up to 20 d. The numbers as predicted by the SAIF method showed good correlation with counts derived by plating techniques (r2 = 0.78; rsd = +/- 0.42). The application of the SAIF technique for the rapid detection of Y. enterocolitica serotype O:3 from meat is discussed.
本研究检测了小肠结肠炎耶尔森氏菌O:3血清型的黏附动力学，以确定使用一种新型表面黏附技术从肉类增菌系统中分离该菌的最佳条件。将碎牛肉接种初始浓度为10 cfu g-1的小肠结肠炎耶尔森氏菌，并在25℃的增菌肉汤中培养。通过表面黏附从富集样品的聚碳酸酯膜上回收耶尔森氏菌，并用免疫荧光显微镜计数。表面黏附免疫荧光技术（SAIF）的最低检测限约为4.0 - 4.5 log10 cfu ml-1，并且在小肠结肠炎耶尔森氏菌选择性琼脂（CIN）平板计数法估算的增菌肉汤中耶尔森氏菌数量与SAIF法测定的数量之间具有良好的相关性（r2 = 0.94；rsd = +/- 0.21）。SAIF计数与平板计数的推导回归方程用于预测在0℃储存长达20天的加标肉样中小肠结肠炎耶尔森氏菌的数量。SAIF法预测的数量与平板计数法得到的计数具有良好的相关性（r2 = 0.78；rsd = +/- 0.42）。本文讨论了SAIF技术在从肉类中快速检测小肠结肠炎耶尔森氏菌O:3血清型中的应用。
