Lecadet M M, Frachon E, Dumanoir V C, Ripouteau H, Hamon S, Laurent P, Thiéry I
Unité des Bactéries Entomopathogènes, Institut Pasteur, Paris, France.
J Appl Microbiol. 1999 Apr;86(4):660-72. doi: 10.1046/j.1365-2672.1999.00710.x.
The classification of Bacillus thuringiensis strains has been revised and updated based on flagellar antigens which have been in use for many years. Sixty-nine serotypes and 13 sub-antigenic groups have now been identified, giving 82 serovars among the 3500 B. thuringiensis isolates of the IEBC Collection. The number of serovars has gradually increased with the total number of strains. The biochemical characters used have also been investigated and their value assessed for identification of B. thuringiensis at the subspecies level. A crystal analysis was carried out in terms of morphology, delta-endotoxin profiles and larvicidal activity for the newly identified serovars. It was found that atypical crystals, some with novel components, are becoming more common. No insect susceptible to these serovars has been discovered among known target species. The number of cross-reacting H-antigens among B. cereus strains is increasing and may be of biological significance.
苏云金芽孢杆菌菌株的分类已根据已使用多年的鞭毛抗原进行了修订和更新。现已鉴定出69个血清型和13个亚抗原组，在国际昆虫学研究中心（IEBC）收集的3500株苏云金芽孢杆菌分离株中共有82个血清变种。血清变种的数量随着菌株总数的增加而逐渐增多。还对所使用的生化特性进行了研究，并评估了它们在亚种水平上鉴定苏云金芽孢杆菌的价值。对新鉴定的血清变种进行了晶体形态、δ-内毒素谱和杀幼虫活性方面的分析。结果发现，非典型晶体，有些含有新成分，正变得越来越普遍。在已知的目标物种中尚未发现对这些血清变种敏感的昆虫。蜡样芽孢杆菌菌株之间交叉反应的H抗原数量正在增加，可能具有生物学意义。
