Murakami T, Hiraoka K, Mikami T, Matsumoto T, Katagiri S, Shinagawa K, Suzuki M
Department of Microbiology, Tohoku College of Pharmacy, Miyagi, Japan.
FEMS Microbiol Lett. 1993 Mar 1;107(2-3):179-83. doi: 10.1111/j.1574-6968.1993.tb06027.x.
Flagellar antigen of Bacillus cereus H.1 was purified and tested for serodiagnostic antigen by ELISA. The antibody against the flagellar antigen of B. cereus H.1 reacted not only with the homologous specific antigen but also reacted with the flagellar antigens of 23 strains of B. cereus. This common flagellar antigen of B. cereus was found to be due to 61-kDa protein by SDS-PAGE and immunoblot assay. Monoclonal antibody H15A5 against common antigenic epitope of B. cereus also reacted with flagellar antigens of 21 strains of Bacillus thuringiensis by ELISA. This monoclonal antibody reacted with the 61-kDa protein of the flagella of B. cereus H.1 and H.2 and B. thuringiensis Kurstaki HD1, Alesti and Aizawai juroi by immunoblot analysis. These results indicated that the common antigenic epitope of the 61-kDa protein existed in the flagella both of B. cereus and B. thuringiensis.
蜡样芽孢杆菌H.1的鞭毛抗原被纯化，并通过酶联免疫吸附测定（ELISA）作为血清诊断抗原进行检测。抗蜡样芽孢杆菌H.1鞭毛抗原的抗体不仅与同源特异性抗原发生反应，还与23株蜡样芽孢杆菌的鞭毛抗原发生反应。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和免疫印迹分析发现，蜡样芽孢杆菌的这种共同鞭毛抗原是由61 kDa的蛋白质引起的。通过ELISA，针对蜡样芽孢杆菌共同抗原表位的单克隆抗体H15A5也与21株苏云金芽孢杆菌的鞭毛抗原发生反应。通过免疫印迹分析，该单克隆抗体与蜡样芽孢杆菌H.1和H.2以及苏云金芽孢杆菌库尔斯塔克HD1、阿莱斯特和艾扎瓦juroi鞭毛的61 kDa蛋白质发生反应。这些结果表明，61 kDa蛋白质的共同抗原表位存在于蜡样芽孢杆菌和苏云金芽孢杆菌的鞭毛中。
