Dewez J L, Doren A, Schneider Y J, Rouxhet P G
Unité de Chimie des Interfaces and Research Center for Advanced Materials, Université catholique de Louvain, Louvain-La-Neuve, Belgium.
Biomaterials. 1999 Mar;20(6):547-59. doi: 10.1016/s0142-9612(98)00207-5.
The adhesion of Hep G2 cells was investigated using different substrata (commercial substrata, polystyrene modified by oxygen or ammonia plasma discharge), the surface properties of which were characterized (surface chemical composition, water contact angle, zeta potential). Some substrata were pre-conditioned with solutions of extracellular matrix (ECM) protein (collagen, laminin, fibronectin), solutions of albumin or polylysin, fetal calf serum or culture medium. The culture medium contained the surfactant Pluronic F68; cycloheximide was added in certain tests to inhibit protein synthesis. Cells spread within 1.5 h provided ECM proteins were present at the surface. Adsorption of ECM proteins was subject to competition with adsorption of Pluronic F68. When the substratum was exposed simultaneously to ECM protein and Pluronic F68, either by pre-conditioning or through protein cell secretion, a weaker substratum hydrophobicity favored adsorption of the proteins and subsequent cell adhesion. On the other hand, when ECM proteins were pre-adsorbed, they were not displaced by Pluronic F68 and cell adhesion was not influenced by substratum hydrophobicity. When ECM proteins were present, no difference was observed between substrata of similar hydrophobicity carrying positive or negative charges, respectively. In absence of ECM proteins, the presence of cationic sites at the substratum surface (NH3 plasma treatment, adsorption of polylysine) allowed cell attachment but no spreading within 1.5 h.
使用不同的基质（商业基质、经氧或氨等离子体放电改性的聚苯乙烯）研究了Hep G2细胞的黏附情况，并对其表面性质（表面化学成分、水接触角、zeta电位）进行了表征。一些基质用细胞外基质（ECM）蛋白（胶原蛋白、层粘连蛋白、纤连蛋白）溶液、白蛋白或聚赖氨酸溶液、胎牛血清或培养基进行预处理。培养基中含有表面活性剂普朗尼克F68；在某些试验中添加了环己酰亚胺以抑制蛋白质合成。如果表面存在ECM蛋白，细胞会在1.5小时内铺展。ECM蛋白的吸附会与普朗尼克F68的吸附产生竞争。当基质通过预处理或通过蛋白质细胞分泌同时暴露于ECM蛋白和普朗尼克F68时，较弱的基质疏水性有利于蛋白质的吸附和随后的细胞黏附。另一方面，当ECM蛋白被预先吸附时，它们不会被普朗尼克F68取代，并且细胞黏附不受基质疏水性的影响。当存在ECM蛋白时，分别带有正电荷或负电荷的类似疏水性基质之间未观察到差异。在没有ECM蛋白的情况下，基质表面的阳离子位点（NH3等离子体处理或聚赖氨酸吸附）允许细胞附着，但在1.5小时内不会铺展。
