Dewez J L, Schneider Y J, Rouxhet P G
Unité de Chimie des Interfaces, Université Catholique de Louvain, Louvain-La-Neuve, Belgium.
J Biomed Mater Res. 1996 Mar;30(3):373-83. doi: 10.1002/(SICI)1097-4636(199603)30:3<373::AID-JBM12>3.0.CO;2-L.
The influence of substratum surface hydrophilicity and of a surfactant on human epithelial cell adhesion and protein adsorption was investigated. Therefore, tissue culture grade polystyrene (TCPS) and bacteriological grade polystyrene (BGPS) substrata were treated with different media, with or without Pluronic F68 [a poly(ethylene oxide) and poly(propylene oxide) triblock copolymer surfactant], and with or without type I collagen as a typical extracellular matrix protein. The conditioned substrata were submitted to XPS analysis and assayed for cell adhesion by inoculating Hep G2 cells in a chemically defined nutritive medium. The presence of collagen at the substratum surface is required to obtain attachment and spreading of Hep G2 cells. With PS substrata, treating with a solution of collagen does not promote cell adhesion if the solution contains Pluronic; XPS data show that this is due either to prevention of collagen adsorption or to its desorption by rinsing. With less hydrophobic TCPS substrata, the presence of Pluronic in the conditioning solution does not preclude cell adhesion, nor collagen adsorption. The effect of BGPS and TCPS substrata on Hep G2 cell adhesion is thus mediated by the presence of a surfactant that affects the adsorption of collagen.
研究了基质表面亲水性和表面活性剂对人上皮细胞黏附及蛋白质吸附的影响。因此，用不同培养基处理组织培养级聚苯乙烯（TCPS）和细菌学级聚苯乙烯（BGPS）基质，培养基中添加或不添加普朗尼克F68（一种聚环氧乙烷和聚环氧丙烷三嵌段共聚物表面活性剂），添加或不添加作为典型细胞外基质蛋白的I型胶原蛋白。对预处理后的基质进行X射线光电子能谱（XPS）分析，并通过在化学成分明确的营养培养基中接种Hep G2细胞来检测细胞黏附情况。基质表面需要存在胶原蛋白才能使Hep G2细胞附着和铺展。对于聚苯乙烯（PS）基质，如果胶原蛋白溶液中含有普朗尼克，用该溶液处理不会促进细胞黏附；XPS数据表明，这要么是由于阻止了胶原蛋白的吸附，要么是由于冲洗导致其解吸。对于疏水性较小的TCPS基质，预处理溶液中普朗尼克的存在并不妨碍细胞黏附，也不影响胶原蛋白的吸附。因此，BGPS和TCPS基质对Hep G2细胞黏附的影响是由影响胶原蛋白吸附的表面活性剂介导的。
