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两栖类幼体红细胞的体外培养。

In vitro culture of larval amphibian erythroblasts.

作者信息

Duprat A M, Flavin M

出版信息

Experientia. 1976 Dec 15;32(12):1587-9. doi: 10.1007/BF01924468.

DOI:10.1007/BF01924468
PMID:1021459
Abstract

A larval erythroblast culture method is described. By this method, it is possible to cultivate for several weeks a homogeneous population of cells (5-10(5) cells/ml medium on average after 4 or 5 days of culture), which are relatively synchronous with regard to their state of differentiation.

摘要

本文描述了一种幼虫成红细胞培养方法。通过这种方法,可以培养出均匀的细胞群体数周(培养4或5天后,平均每毫升培养基中有5-10(5)个细胞),这些细胞在分化状态方面相对同步。

相似文献

1
In vitro culture of larval amphibian erythroblasts.两栖类幼体红细胞的体外培养。
Experientia. 1976 Dec 15;32(12):1587-9. doi: 10.1007/BF01924468.
2
[ON THE MECHANISM OF THE ANAPHASIC SEPARATION OF CHROMATIDS AT THE LEVEL OF THE CENTROMERIC ZONE. MICRO-CINEMATOGRAPHIC RESEARCH ON ERYTHROBLASTS IN THE VITAL STATE].[着丝粒区水平染色单体后期分离的机制。活态成红细胞的显微电影摄影研究]
Haematologica. 1963;48:921-9.
3
In vitro morphogenesis of amphibian erythroblasts.两栖类成红细胞的体外形态发生
Cell Biol Int. 2001;25(12):1229-36. doi: 10.1006/cbir.2001.0800.
4
ELECTRON MICROSCOPE OBSERVATIONS ON MITOTIC CHROMOSOMES IN ERYTHROBLASTS OF THE NEWT, TRITURUS CRISTATUS CRISTATUS.对欧螈(Triturus cristatus cristatus)成红细胞有丝分裂染色体的电子显微镜观察
Nature. 1964 Aug 29;203:990-2. doi: 10.1038/203990b0.
5
[In vitro culture of human erythroblast precursors].[人成红细胞前体的体外培养]
Pathol Biol (Paris). 1981 Feb;29(2):69-72.
6
[In vitro studies on amplification of rat erythropoiesis (author's transl)].大鼠红细胞生成扩增的体外研究(作者译)
Nouv Rev Fr Hematol Blood Cells. 1977 May-Jun;18(3):627-31.
7
Under HEMA conditions, self-replication of human erythroblasts is limited by autophagic death.在 HEMA 条件下,人红细胞的自我复制受到自噬性死亡的限制。
Blood Cells Mol Dis. 2011 Oct 15;47(3):182-97. doi: 10.1016/j.bcmd.2011.06.001. Epub 2011 Jul 20.
8
Membrane differentiation in human erythroid cells: unique profiles of cell surface glycoproteins expressed in erythroblasts in vitro from three ontogenic stages.人类红细胞系细胞中的膜分化:来自三个个体发育阶段的体外成红细胞所表达的细胞表面糖蛋白的独特谱型。
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3474-8. doi: 10.1073/pnas.77.6.3474.
9
Splenic erythroblasts in anemia-inducing Friend disease: a source of cells for studies of erythropoietin-mediated differentiation.贫血诱导型弗瑞德病中的脾成红细胞:用于促红细胞生成素介导分化研究的细胞来源。
J Cell Physiol. 1984 Dec;121(3):526-32. doi: 10.1002/jcp.1041210311.
10
[Comparative investigation of the non-histone proteins of chromatin from pigeon erythroblasts and erythrocytes].[鸽成红细胞和红细胞染色质非组蛋白的比较研究]
Mol Biol (Mosk). 1976 May-Jun;10(2):593-9.

本文引用的文献

1
AMPHIBIAN CELL CULTURE: PERMANENT CELL LINE FROM THE BULLFROG (RANA CATESBEIANA).两栖动物细胞培养:源自牛蛙(北美牛蛙)的永久细胞系。
Science. 1964 Jun 26;144(3626):1578-80. doi: 10.1126/science.144.3626.1578.
2
THE GROWTH AND MAINTENANCE OF TISSUE-CELL CULTURES IN FREE GAS EXCHANGE WITH THE ATMOSPHERE.与大气进行自由气体交换的组织细胞培养物的生长与维持
Am J Hyg. 1963 Sep;78:173-80. doi: 10.1093/oxfordjournals.aje.a120336.
3
Differentiation of cells of the Rana pipiens gastrula in unconditioned medium.爪蟾原肠胚细胞在未 conditioned 培养基中的分化。 注:这里原文中“unconditioned medium”的“conditioned”可能拼写有误,正常应该是“conditioned medium”,意思是“条件培养基”,如果是“unconditioned medium”就是“非条件培养基”,但仅根据现有文本准确翻译就是上述译文。
J Embryol Exp Morphol. 1959 Jun;7:210-22.
4
Haemoglobin synthesis in vitro by erythrocytes from Xenopus laevis.
Comp Biochem Physiol. 1969 Sep 1;30(5):825-34. doi: 10.1016/0010-406x(69)90037-1.
5
Erythropoietic cell cultures from chick embryos.来自鸡胚的红细胞生成细胞培养物。
J Cell Biol. 1972 Jul;54(1):98-106. doi: 10.1083/jcb.54.1.98.
6
Isolation of two erythropoietic cell populations from the early chick blastodisc and the further resolution of one into two essential subpopulations.从早期鸡胚盘分离出两个红细胞生成细胞群体,并将其中一个进一步解析为两个基本亚群。
Exp Cell Res. 1974 Sep;88(1):143-52. doi: 10.1016/0014-4827(74)90628-4.
7
The blood as an erythropoietic organ in anaemic Xenopus.
Experientia. 1974 Sep 15;30(9):1083-5. doi: 10.1007/BF01939024.
8
Hemoglobin synthesis in isolated erythroid colonies from the chick embryo.鸡胚分离红系集落中的血红蛋白合成
Dev Biol. 1976 Apr;49(2):556-62. doi: 10.1016/0012-1606(76)90198-6.
9
Differentiation of red blood cells in vitro.红细胞的体外分化。
Science. 1975 Oct 31;190(4213):471-3. doi: 10.1126/science.1080882.
10
Erythropoiesis in cultures of dispersed cell preparations from early chick blastodiscs.早期鸡胚盘分散细胞制备物培养中的红细胞生成
Exp Cell Res. 1975 Dec;96(2):432-5. doi: 10.1016/0014-4827(75)90281-5.