Fukuda M, Fukuda M N, Papayannopoulou T, Hakomori S
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3474-8. doi: 10.1073/pnas.77.6.3474.
HUMAN ERYTHROBLASTS IN CULTURE, IRRESPECTIVE OF THE ONTOGENIC STAGE OF THEIR PROGENITORS, ARE CHARACTERIZED BY: (i) the barely detectable amount of band 3 glycoprotein, (ii) the presence of two glycoproteins with molecular weights 105,000 and 95,000, (iii) the high concentration of glycophorin, and (iv) a minimum quantity of the carbohydrate chain susceptible to endo-beta-galactosidase ("polylactosaminoglycan"). In contrast, mature erythrocytes, whether of fetal, neonatal, or adult origin, are characterized by a high concentration of band 3 glycoprotein, polylactosaminoglycan, and glycophorins, but do not contain 105- and 95-kilodalton-glycoproteins. Thus, the process of erythroid maturation from erythroblasts to erythrocytes is accompanied by the appearance of band 3, the disappearance of 105- and 95-kilodalton glycoproteins, and a great increase in the quantity of polylactosaminoglycan. The structure of polylactosaminoglycan may not be different between mature erythrocytes and erythroblasts from the same ontogenic stage, but it is distinctively different from one stage to the other. The profiles of oligosaccharides released by endo-beta-galactosidase and immunofluorescence studies with anti-Ii antibodies indicated that a linear polylactosaminoglycan structure was present in erythroblasts as well as in erythrocytes of the fetal and newborn stage, whereas a branched polylactosaminoglycan structure was present in erythroblasts as well as erythrocytes of adult blood. Thus, two membrane characteristics are closely associated with the process of erythroid cell development; one-the membrane proteins band 3, band 4.5, and 95- and 105-kilodalton glycoproteins-determines the degree of maturation, and the other-polylactosaminoglycan-may determine the ontogenic stage of the erythroblast progenitors.
培养中的人成红细胞，无论其祖细胞的个体发育阶段如何，都具有以下特征：(i) 几乎检测不到的带3糖蛋白量；(ii) 存在分子量为105,000和95,000的两种糖蛋白；(iii) 血型糖蛋白浓度高；(iv) 对内切β-半乳糖苷酶敏感的碳水化合物链（“多乳糖胺聚糖”）量最少。相比之下，成熟红细胞，无论来源于胎儿、新生儿还是成人，其特征是带3糖蛋白、多乳糖胺聚糖和血型糖蛋白浓度高，但不含有105千道尔顿和95千道尔顿的糖蛋白。因此，从成红细胞到红细胞的红细胞成熟过程伴随着带3的出现、105千道尔顿和95千道尔顿糖蛋白的消失以及多乳糖胺聚糖量的大幅增加。同一发育阶段的成熟红细胞和成红细胞之间，多乳糖胺聚糖的结构可能没有差异，但在不同发育阶段之间则明显不同。内切β-半乳糖苷酶释放的寡糖谱以及用抗Ii抗体进行的免疫荧光研究表明，胎儿和新生儿阶段的成红细胞以及红细胞中存在线性多乳糖胺聚糖结构，而成人血液的成红细胞以及红细胞中存在分支多乳糖胺聚糖结构。因此，两种膜特征与红细胞发育过程密切相关；一种是膜蛋白带3、带4.5以及95千道尔顿和105千道尔顿糖蛋白，它们决定成熟程度，另一种是多乳糖胺聚糖，它可能决定成红细胞祖细胞的个体发育阶段。
