Meyer K, Keil M, Naldrett M J
Shell International Renewables, Forestry Research Unit, East Malling, West Malling, UK.
FEBS Lett. 1999 Mar 26;447(2-3):171-8. doi: 10.1016/s0014-5793(99)00280-x.
A gene encoding an antifreeze protein (AFP) was isolated from carrot (Daucus carota) using sequence information derived from the purified protein. The carrot AFP is highly similar to the polygalacturonase inhibitor protein (PGIP) family of apoplastic plant leucine-rich repeat (LRR) proteins. Expression of the AFP gene is rapidly induced by low temperatures. Furthermore, expression of the AFP gene in transgenic Arabidopsis thaliana plants leads to an accumulation of antifreeze activity. Our findings suggest that a new type of plant antifreeze protein has recently evolved from PGIPs.
利用从纯化蛋白获得的序列信息，从胡萝卜（Daucus carota）中分离出一种编码抗冻蛋白（AFP）的基因。胡萝卜AFP与质外体富含亮氨酸重复序列（LRR）的植物多聚半乳糖醛酸酶抑制蛋白（PGIP）家族高度相似。AFP基因的表达受低温快速诱导。此外，AFP基因在转基因拟南芥植物中的表达导致抗冻活性的积累。我们的研究结果表明，一种新型的植物抗冻蛋白最近从PGIPs进化而来。
