Lacassie E, Ragot S, Gaulier J M, Marquet P, Lâchatre G
Service de Pharmacologie et Toxicologie, CHRU Dupuytren, Limoges, France.
Acta Clin Belg. 1999;53 Suppl 1:20-4.
A specific and sensitive method for the analysis of 24 antidepressants in human serum was developed using gas chromatography-mass spectrometry (GC/MS). This method allowed the simultaneous determination of antidepressants belonging to different classes: tricyclic antidepressants (TADs), selective serotonin reuptake inhibitors (SSRIs) and selective inhibitors of monoamine oxidase A (IMAOs). Antidepressants were submitted to liquid-liquid extraction at pH 9.5 using a mixture of heptane/isoamyl alcohol (98.5/1.5; v/v) without derivatization. Cyproheptadine was used as the internal standard (IS). Separation was obtained with a nonpolar PTE5 capillary column (30 m x 0.32 mm; film thickness 0.25 micron). Mass spectrometry consisted of electron impact ionisation (70 eV), and full scan acquisition. Extraction recoveries were over 60% for 22 antidepressants and between 35 and 95% for moclobemide and viloxazine. Limits of quantitation ranged from 20 to 100 ng/ml for most of the antidepressants, except for moclobemide and viloxazine for which it was 500 ng/ml. Intra-assay standard deviation was satisfactory. An excellent linearity was observed from the respective LOQs up to 1000 ng/ml for 22 antidepressants and up to 4000 ng/ml for moclobemide and viloxazine.
建立了一种采用气相色谱 - 质谱联用（GC/MS）分析人血清中24种抗抑郁药的特异且灵敏的方法。该方法可同时测定不同类型的抗抑郁药：三环类抗抑郁药（TADs）、选择性5-羟色胺再摄取抑制剂（SSRIs）以及单胺氧化酶A选择性抑制剂（IMAOs）。抗抑郁药在pH 9.5条件下，使用庚烷/异戊醇混合液（98.5/1.5；v/v）进行液 - 液萃取，无需衍生化处理。赛庚啶用作内标（IS）。采用非极性PTE5毛细管柱（30 m×0.32 mm；膜厚0.25微米）进行分离。质谱分析采用电子轰击电离（70 eV）和全扫描采集。22种抗抑郁药的萃取回收率超过60%，吗氯贝胺和维洛沙嗪的萃取回收率在35%至95%之间。大多数抗抑郁药的定量限为20至100 ng/ml，吗氯贝胺和维洛沙嗪的定量限为500 ng/ml。批内标准差令人满意。22种抗抑郁药从各自的定量限到1000 ng/ml，以及吗氯贝胺和维洛沙嗪从定量限到4000 ng/ml均呈现出良好的线性关系。
