Yang S Y, Tsuboi M
Department of Fundamental Science, Iwaki-Meisei University, Iwaki, Fukushima, Japan.
Biospectroscopy. 1999;5(2):93-100. doi: 10.1002/(SICI)1520-6343(1999)5:2<93::AID-BSPY3>3.0.CO;2-X.
The eyespot in the cell of Chlamydomonas reinhardtii has been found to appear as a bright spot under the cross polar setting of a polarizing microscope. This was confirmed by isolating the eyespot from a homogenate of wall-deficient mutant cw-15, and by observing it under a polarizing microscope. Thus, the eyespot was proved to be a strongly birefringent body. Next, gametes (mt+ and mt-) of 137c strain were prepared by cultivating it in a low-nitrogen (NH4Cl) medium. Here, every cell shows only one (and never more than one) birefringent spot. The birefringent eyespot was located always near the surface on the "equator," that is, at the farthest point from the "meridionial" cell-axis that is defined as the bisector of the two flagella projected out from the cell surface. It was shown, in addition, that the optic axis of this birefringent eyespot is oriented in the cell always along the parallel direction of the cell axis defined above. Thus, the polarizing microscopy has been shown to provide a powerful method for in vivo, in situ pursuit of the eyespot of Chlamydomonas.
人们发现，莱茵衣藻细胞中的眼点在偏光显微镜的交叉偏振设置下呈现为一个亮点。通过从细胞壁缺陷型突变体cw - 15的匀浆中分离出眼点，并在偏光显微镜下进行观察，这一点得到了证实。因此，眼点被证明是一个强双折射体。接下来，通过在低氮（氯化铵）培养基中培养137c菌株来制备配子（mt +和mt -）。在此，每个细胞仅显示一个（且从不超过一个）双折射点。双折射眼点总是位于“赤道”表面附近，即距离从细胞表面伸出的两条鞭毛的平分线所定义的“子午线”细胞轴最远的点。此外，还表明这个双折射眼点的光轴在细胞中总是沿着上述定义的细胞轴的平行方向排列。因此，偏光显微镜已被证明是一种在体内、原位追踪莱茵衣藻眼点的有力方法。
