Zhang Y
Inst. of Crop Breeding & Cultivation, CAAS, Beijing, China.
Biochem Biophys Res Commun. 1994 Dec 15;205(2):1025-35. doi: 10.1006/bbrc.1994.2769.
Using anti-rhodopsin antibody, an immunofluorescence method was established in Chlamydomonas reinhardtii in order to locate rhodopsin in the cell. The method showed that rhodopsin is located in the membrane near (or around) the pigmented eyespot in green algae. In synchronized cells, the location of rhodopsin changes regularly during the cell cycle. The fluorescence (F) spot moves from the anterior region toward the posterior region of the chloroplast (cp) in the light period. Following cell division, the F spots reappear in each daughter cell whether the daughter cells are released from the mother cell wall or not. The F spot is located opposite the site of the last cleavage furrow whether the cell has divided once, twice, or three times. The F spot moves back toward the anterior region from the equatorial plane after division in the dark period. In white algae eyespot-deficient mutants, rhodopsin does not depend on the presence of the eyespot. The results further showed that rhodopsin resides in the plasmalemma.
利用抗视紫红质抗体，在莱茵衣藻中建立了一种免疫荧光方法，以便在细胞中定位视紫红质。该方法表明，视紫红质位于绿藻中有色素的眼点附近（或周围）的膜中。在同步化细胞中，视紫红质的位置在细胞周期中定期变化。在光照期，荧光（F）点从叶绿体（cp）的前部区域移向后部区域。细胞分裂后，无论子细胞是否从母细胞壁释放，F点都会在每个子细胞中重新出现。无论细胞分裂一次、两次还是三次，F点都位于最后一次分裂沟的对面。在黑暗期分裂后，F点从赤道平面向后部区域移动。在白色藻类眼点缺陷突变体中，视紫红质不依赖于眼点的存在。结果进一步表明，视紫红质存在于质膜中。
